Detailed information of TA system TA231693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 77499..78136
• Replicon: chromosome
• Accession: NZ_CP090714
• Organism: Burkholderia multivorans strain 2015Y70629342III

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z12_RS00340
• Protein ID: WP_053296685.1
• Coordinates: 77735..78136 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z12_RS00335
• Protein ID: WP_006408845.1
• Coordinates: 77499..77735 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z12_RS00315 (L0Z12_00315)	72661..73203	+	543	WP_088923753.1	hypothetical protein	-
L0Z12_RS00320 (L0Z12_00320)	73374..74405	+	1032	WP_105751888.1	helix-turn-helix domain-containing protein	-
L0Z12_RS00325 (L0Z12_00325)	74524..75612	-	1089	WP_105751887.1	ADP-heptose--LPS heptosyltransferase	-
L0Z12_RS00330 (L0Z12_00330)	75609..77312	-	1704	WP_105751886.1	thiamine pyrophosphate-binding protein	-
L0Z12_RS00335 (L0Z12_00335)	77499..77735	+	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z12_RS00340 (L0Z12_00340)	77735..78136	+	402	WP_053296685.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z12_RS00345 (L0Z12_00345)	78258..79646	-	1389	WP_006408285.1	L-serine ammonia-lyase	-
L0Z12_RS00350 (L0Z12_00350)	79673..80815	-	1143	WP_070555436.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14963.17 Da
• Isoelectric Point: 5.7429

>T231693 WP_053296685.1 NZ_CP090714:77735-78136 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCANPARERRNLAALIEDIPVAPFDGAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231693 NZ_CP126943:86493-86909 [Escherichia coli O1:K1:H7]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231693 WP_006408845.1 NZ_CP090714:77499-77735 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231693 NZ_CP126943:86266-86496 [Escherichia coli O1:K1:H7]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1