Detailed information of TA system TA231691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1656359..1657014
• Replicon: chromosome
• Accession: NZ_CP090708
• Organism: Burkholderia multivorans strain 2016Y70836300

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z15_RS23355
• Protein ID: WP_006403072.1
• Coordinates: 1656359..1656778 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z15_RS23360
• Protein ID: WP_006403071.1
• Coordinates: 1656775..1657014 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z15_RS23330 (L0Z15_23330)	1651528..1652322	+	795	WP_006403076.1	AraC family transcriptional regulator	-
L0Z15_RS23335 (L0Z15_23335)	1652749..1653699	+	951	WP_105752205.1	DMT family transporter	-
L0Z15_RS23340 (L0Z15_23340)	1653894..1654469	+	576	WP_006403074.1	hypothetical protein	-
L0Z15_RS23345 (L0Z15_23345)	1654563..1654697	-	135	WP_006396654.1	entericidin A/B family lipoprotein	-
L0Z15_RS23350 (L0Z15_23350)	1654930..1656225	+	1296	WP_035487510.1	aspartate carbamoyltransferase	-
L0Z15_RS23355 (L0Z15_23355)	1656359..1656778	-	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z15_RS23360 (L0Z15_23360)	1656775..1657014	-	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z15_RS23365 (L0Z15_23365)	1657217..1658023	-	807	WP_105799145.1	IS5 family transposase	-
L0Z15_RS23370 (L0Z15_23370)	1658177..1660423	+	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z15_RS23375 (L0Z15_23375)	1660427..1661182	+	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z15_RS23380 (L0Z15_23380)	1661179..1661862	+	684	WP_006396647.1	Fe2+-dependent dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1657217..1658023	806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231691 WP_006403072.1 NZ_CP090708:c1656778-1656359 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231691 NZ_CP126943:37687-38085 [Escherichia coli O1:K1:H7]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231691 WP_006403071.1 NZ_CP090708:c1657014-1656775 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231691 NZ_CP126943:37460-37687 [Escherichia coli O1:K1:H7]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6