Detailed information of TA system TA231690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1545269..1547016
• Replicon: chromosome
• Accession: NZ_CP090708
• Organism: Burkholderia multivorans strain 2016Y70836300

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B9BHC0
• Locus tag: L0Z15_RS22815
• Protein ID: WP_006403210.1
• Coordinates: 1546120..1547016 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: L0Z15_RS22810
• Protein ID: WP_006396780.1
• Coordinates: 1545269..1546123 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z15_RS22790 (L0Z15_22790)	1540440..1541930	-	1491	WP_105799370.1	aldehyde dehydrogenase	-
L0Z15_RS22795 (L0Z15_22795)	1541992..1542822	-	831	WP_006403212.1	aspartate dehydrogenase	-
L0Z15_RS22800 (L0Z15_22800)	1542815..1544482	-	1668	WP_053298420.1	thiamine pyrophosphate-binding protein	-
L0Z15_RS22805 (L0Z15_22805)	1544475..1545272	-	798	WP_006409174.1	SDR family oxidoreductase	-
L0Z15_RS22810 (L0Z15_22810)	1545269..1546123	-	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
L0Z15_RS22815 (L0Z15_22815)	1546120..1547016	-	897	WP_006403210.1	alpha/beta fold hydrolase	Toxin
L0Z15_RS22820 (L0Z15_22820)	1547035..1547562	-	528	WP_006396778.1	cupin domain-containing protein	-
L0Z15_RS22825 (L0Z15_22825)	1547590..1548360	-	771	WP_105752056.1	SDR family oxidoreductase	-
L0Z15_RS22830 (L0Z15_22830)	1548353..1549423	-	1071	WP_065496357.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L0Z15_RS22835 (L0Z15_22835)	1549471..1549782	-	312	WP_053298417.1	hypothetical protein	-
L0Z15_RS22840 (L0Z15_22840)	1549784..1550101	-	318	WP_053298416.1	non-heme iron oxygenase ferredoxin subunit	-
L0Z15_RS22845 (L0Z15_22845)	1550538..1551617	+	1080	WP_006409825.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30544.80 Da
• Isoelectric Point: 8.0446

>T231690 WP_006403210.1 NZ_CP090708:c1547016-1546120 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQQAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDAFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T231690 NZ_CP126943:c28731-28582 [Escherichia coli O1:K1:H7]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT231690 WP_006396780.1 NZ_CP090708:c1546123-1545269 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT231690 NZ_CP126943:28775-28836 [Escherichia coli O1:K1:H7]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: B9BHC0

Antitoxin

• AlphaFold DB: B9AZJ2