Detailed information of TA system TA231686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3299645..3300282
• Replicon: chromosome
• Accession: NZ_CP090707
• Organism: Burkholderia multivorans strain 2016Y70836300

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z15_RS15500
• Protein ID: WP_035489546.1
• Coordinates: 3299645..3300046 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z15_RS15505
• Protein ID: WP_006408845.1
• Coordinates: 3300046..3300282 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z15_RS15490 (L0Z15_15490)	3296966..3298108	+	1143	WP_053296686.1	alginate lyase family protein	-
L0Z15_RS15495 (L0Z15_15495)	3298135..3299523	+	1389	WP_105751885.1	L-serine ammonia-lyase	-
L0Z15_RS15500 (L0Z15_15500)	3299645..3300046	-	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z15_RS15505 (L0Z15_15505)	3300046..3300282	-	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z15_RS15510 (L0Z15_15510)	3300470..3302173	+	1704	WP_105751886.1	thiamine pyrophosphate-binding protein	-
L0Z15_RS15515 (L0Z15_15515)	3302170..3303258	+	1089	WP_105751887.1	ADP-heptose--LPS heptosyltransferase	-
L0Z15_RS15520 (L0Z15_15520)	3303377..3304408	-	1032	WP_105751888.1	helix-turn-helix domain-containing protein	-
L0Z15_RS15525 (L0Z15_15525)	3304579..3305121	-	543	WP_088923753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231686 WP_035489546.1 NZ_CP090707:c3300046-3299645 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231686 NZ_CP126942:4615277-4615654 [Escherichia coli O1:K1:H7]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231686 WP_006408845.1 NZ_CP090707:c3300282-3300046 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231686 NZ_CP126942:4614820-4615188 [Escherichia coli O1:K1:H7]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1