Detailed information of TA system TA231685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2066536..2067236
• Replicon: chromosome
• Accession: NZ_CP090707
• Organism: Burkholderia multivorans strain 2016Y70836300

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8E2RY82
• Locus tag: L0Z15_RS09600
• Protein ID: WP_070553238.1
• Coordinates: 2066536..2066913 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z15_RS09605
• Protein ID: WP_006405635.1
• Coordinates: 2066913..2067236 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z15_RS09575 (L0Z15_09575)	2062067..2062516	+	450	WP_105751509.1	VOC family protein	-
L0Z15_RS09580 (L0Z15_09580)	2062630..2064180	-	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z15_RS09585 (L0Z15_09585)	2064177..2064410	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z15_RS09590 (L0Z15_09590)	2064607..2065524	-	918	WP_053296988.1	spermidine synthase	-
L0Z15_RS09595 (L0Z15_09595)	2065752..2066006	+	255	WP_059940739.1	hypothetical protein	-
L0Z15_RS09600 (L0Z15_09600)	2066536..2066913	+	378	WP_070553238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z15_RS09605 (L0Z15_09605)	2066913..2067236	+	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z15_RS09610 (L0Z15_09610)	2067259..2067750	-	492	WP_006398691.1	DNA-deoxyinosine glycosylase	-
L0Z15_RS09615 (L0Z15_09615)	2067747..2068361	-	615	WP_006398690.1	chorismate lyase	-
L0Z15_RS09620 (L0Z15_09620)	2068370..2070268	-	1899	WP_059875776.1	molecular chaperone HtpG	-
L0Z15_RS09625 (L0Z15_09625)	2070427..2071887	-	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13507.49 Da
• Isoelectric Point: 9.3081

>T231685 WP_070553238.1 NZ_CP090707:2066536-2066913 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 378 bp

>T231685 NZ_CP126942:4351346-4351603 [Escherichia coli O1:K1:H7]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231685 WP_006405635.1 NZ_CP090707:2066913-2067236 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231685 NZ_CP126942:4351615-4352160 [Escherichia coli O1:K1:H7]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: B9BQB8