Detailed information of TA system TA231684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 736557..737190
• Replicon: chromosome
• Accession: NZ_CP090707
• Organism: Burkholderia multivorans strain 2016Y70836300

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z15_RS03430
• Protein ID: WP_105767892.1
• Coordinates: 736777..737190 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z15_RS03425
• Protein ID: WP_085545763.1
• Coordinates: 736557..736790 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z15_RS03405 (L0Z15_03405)	732172..733311	+	1140	WP_105751490.1	nucleoside triphosphate pyrophosphohydrolase family protein	-
L0Z15_RS03410 (L0Z15_03410)	733308..733970	+	663	WP_105751491.1	uracil-DNA glycosylase	-
L0Z15_RS03420 (L0Z15_03420)	735702..736250	-	549	WP_249485854.1	phospholipase D family protein	-
L0Z15_RS03425 (L0Z15_03425)	736557..736790	+	234	WP_085545763.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Z15_RS03430 (L0Z15_03430)	736777..737190	+	414	WP_105767892.1	PIN domain-containing protein	Toxin
L0Z15_RS03435 (L0Z15_03435)	737225..737587	+	363	WP_105767921.1	DUF3742 family protein	-
L0Z15_RS03440 (L0Z15_03440)	737709..738968	-	1260	WP_249458451.1	IS256-like element IS1356 family transposase	-
L0Z15_RS03445 (L0Z15_03445)	739033..740466	-	1434	Protein_672	conjugal transfer protein TraG N-terminal domain-containing protein	-
L0Z15_RS03450 (L0Z15_03450)	740479..740808	-	330	WP_105767975.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	731542..744771	13229
	-	inside	IScluster/Tn	-	-	734539..738968	4429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15293.73 Da
• Isoelectric Point: 6.0823

>T231684 WP_105767892.1 NZ_CP090707:736777-737190 [Burkholderia multivorans]
MSGADKAKVFVDSNVVLYLLSGDAAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVDQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Nucleotide

• Length: 414 bp

>T231684 NZ_CP126942:4300346-4300687 [Escherichia coli O1:K1:H7]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGGTTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.97 Da
• Isoelectric Point: 7.0164

>AT231684 WP_085545763.1 NZ_CP090707:736557-736790 [Burkholderia multivorans]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRHFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT231684 NZ_CP126942:c4300351-4300275 [Escherichia coli O1:K1:H7]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCAGGTGTGTTGGCGCACATCCGCACCGCGCT