Detailed information of TA system TA231681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1565194..1565849
• Replicon: chromosome
• Accession: NZ_CP090705
• Organism: Burkholderia multivorans strain 2016Y70888756VI

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z41_RS25465
• Protein ID: WP_006403072.1
• Coordinates: 1565194..1565613 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BGX6
• Locus tag: L0Z41_RS25470
• Protein ID: WP_006403071.1
• Coordinates: 1565610..1565849 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z41_RS25440 (L0Z41_25440)	1560363..1561157	+	795	WP_006403076.1	AraC family transcriptional regulator	-
L0Z41_RS25445 (L0Z41_25445)	1561584..1562534	+	951	WP_105752205.1	DMT family transporter	-
L0Z41_RS25450 (L0Z41_25450)	1562729..1563304	+	576	WP_006403074.1	hypothetical protein	-
L0Z41_RS25455 (L0Z41_25455)	1563398..1563532	-	135	WP_006396654.1	entericidin A/B family lipoprotein	-
L0Z41_RS25460 (L0Z41_25460)	1563765..1565060	+	1296	WP_035487510.1	aspartate carbamoyltransferase	-
L0Z41_RS25465 (L0Z41_25465)	1565194..1565613	-	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z41_RS25470 (L0Z41_25470)	1565610..1565849	-	240	WP_006403071.1	Arc family DNA-binding protein	Antitoxin
L0Z41_RS25475 (L0Z41_25475)	1566153..1568399	+	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z41_RS25480 (L0Z41_25480)	1568403..1569158	+	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z41_RS25485 (L0Z41_25485)	1569155..1569838	+	684	WP_006396647.1	Fe2+-dependent dioxygenase	-
L0Z41_RS25490 (L0Z41_25490)	1569852..1570055	-	204	WP_006403067.1	hypothetical protein	-
L0Z41_RS25495 (L0Z41_25495)	1570644..1570787	+	144	WP_006409932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231681 WP_006403072.1 NZ_CP090705:c1565613-1565194 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231681 NZ_CP126942:3693413-3693631 [Escherichia coli O1:K1:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8698.91 Da
• Isoelectric Point: 5.1133

>AT231681 WP_006403071.1 NZ_CP090705:c1565849-1565610 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPISFE

Nucleotide

• Length: 240 bp

>AT231681 NZ_CP126942:3693013-3693387 [Escherichia coli O1:K1:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: B9BGY2

Antitoxin

• AlphaFold DB: B9BGX6