Detailed information of TA system TA231676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2404924..2405624
• Replicon: chromosome
• Accession: NZ_CP090703
• Organism: Burkholderia multivorans strain 2016Y70888756VI

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8E2RY82
• Locus tag: L0Z41_RS11305
• Protein ID: WP_070553238.1
• Coordinates: 2404924..2405301 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: L0Z41_RS11310
• Protein ID: WP_006405635.1
• Coordinates: 2405301..2405624 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z41_RS11280 (L0Z41_11280)	2400455..2400904	+	450	WP_105751509.1	VOC family protein	-
L0Z41_RS11285 (L0Z41_11285)	2401018..2402568	-	1551	WP_006398698.1	sodium:solute symporter family protein	-
L0Z41_RS11290 (L0Z41_11290)	2402565..2402798	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
L0Z41_RS11295 (L0Z41_11295)	2402995..2403912	-	918	WP_053296988.1	spermidine synthase	-
L0Z41_RS11300 (L0Z41_11300)	2404140..2404394	+	255	WP_059940739.1	hypothetical protein	-
L0Z41_RS11305 (L0Z41_11305)	2404924..2405301	+	378	WP_070553238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z41_RS11310 (L0Z41_11310)	2405301..2405624	+	324	WP_006405635.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z41_RS11315 (L0Z41_11315)	2405647..2406138	-	492	WP_006398691.1	DNA-deoxyinosine glycosylase	-
L0Z41_RS11320 (L0Z41_11320)	2406135..2406749	-	615	WP_006398690.1	chorismate lyase	-
L0Z41_RS11325 (L0Z41_11325)	2406758..2408656	-	1899	WP_059875776.1	molecular chaperone HtpG	-
L0Z41_RS11330 (L0Z41_11330)	2408815..2410275	-	1461	WP_006405639.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13507.49 Da
• Isoelectric Point: 9.3081

>T231676 WP_070553238.1 NZ_CP090703:2404924-2405301 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGEK

Nucleotide

• Length: 378 bp

>T231676 NZ_CP126942:2297656-2297758 [Escherichia coli O1:K1:H7]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT231676 WP_006405635.1 NZ_CP090703:2405301-2405624 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT231676 NZ_CP126942:c2297796-2297528 [Escherichia coli O1:K1:H7]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: B9BQB8