Detailed information of TA system TA231675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 77481..78118
• Replicon: chromosome
• Accession: NZ_CP090703
• Organism: Burkholderia multivorans strain 2016Y70888756VI

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z41_RS00340
• Protein ID: WP_035489546.1
• Coordinates: 77717..78118 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z41_RS00335
• Protein ID: WP_006408845.1
• Coordinates: 77481..77717 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z41_RS00315 (L0Z41_00315)	72642..73184	+	543	WP_088923753.1	hypothetical protein	-
L0Z41_RS00320 (L0Z41_00320)	73355..74386	+	1032	WP_105751888.1	helix-turn-helix domain-containing protein	-
L0Z41_RS00325 (L0Z41_00325)	74505..75593	-	1089	WP_105751887.1	ADP-heptose--LPS heptosyltransferase	-
L0Z41_RS00330 (L0Z41_00330)	75590..77293	-	1704	WP_105751886.1	thiamine pyrophosphate-binding protein	-
L0Z41_RS00335 (L0Z41_00335)	77481..77717	+	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z41_RS00340 (L0Z41_00340)	77717..78118	+	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z41_RS00345 (L0Z41_00345)	78240..79628	-	1389	WP_105751885.1	L-serine ammonia-lyase	-
L0Z41_RS00350 (L0Z41_00350)	79655..80797	-	1143	WP_053296686.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231675 WP_035489546.1 NZ_CP090703:77717-78118 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231675 NZ_CP126942:2090035-2090289 [Escherichia coli O1:K1:H7]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231675 WP_006408845.1 NZ_CP090703:77481-77717 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231675 NZ_CP126942:2089787-2090038 [Escherichia coli O1:K1:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1