Detailed information of TA system TA231666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3324388..3325025
• Replicon: chromosome
• Accession: NZ_CP090699
• Organism: Burkholderia cepacia strain 2017Y70952565VI

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2FRJ0
• Locus tag: L0Z01_RS31165
• Protein ID: WP_021164186.1
• Coordinates: 3324624..3325025 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: L0Z01_RS31160
• Protein ID: WP_042978332.1
• Coordinates: 3324388..3324624 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z01_RS31145 (L0Z01_31145)	3319881..3320912	+	1032	WP_042978041.1	helix-turn-helix domain-containing protein	-
L0Z01_RS31150 (L0Z01_31150)	3321194..3322306	-	1113	WP_249452841.1	ADP-heptose--LPS heptosyltransferase	-
L0Z01_RS31155 (L0Z01_31155)	3322303..3324006	-	1704	WP_249452835.1	thiamine pyrophosphate-binding protein	-
L0Z01_RS31160 (L0Z01_31160)	3324388..3324624	+	237	WP_042978332.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z01_RS31165 (L0Z01_31165)	3324624..3325025	+	402	WP_021164186.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z01_RS31170 (L0Z01_31170)	3325093..3326481	-	1389	WP_021164187.1	L-serine ammonia-lyase	-
L0Z01_RS31175 (L0Z01_31175)	3326512..3327648	-	1137	WP_249452833.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15033.14 Da
• Isoelectric Point: 5.2050

>T231666 WP_021164186.1 NZ_CP090699:3324624-3325025 [Burkholderia cepacia]
MPRYMLDTNMCIYLMKHQPEQVAKRFAQCYTGDVVMSAITYAELEYGVAACANAVRERRNLADLIEDIPVAPFDAAAAQA
YGPVREATRERKRDHLDKLIAAHAVSLDVVLVTNNERDFASYPGLRLENWLND

Nucleotide

• Length: 402 bp

>T231666 NZ_CP126941:163813-164211 [Escherichia coli O2:K1:H7]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8926.06 Da
• Isoelectric Point: 5.1651

>AT231666 WP_042978332.1 NZ_CP090699:3324388-3324624 [Burkholderia cepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPVRRPLAGVLEKFAKFAPDFMAEGRGDQEQADREGL

Nucleotide

• Length: 237 bp

>AT231666 NZ_CP126941:163586-163813 [Escherichia coli O2:K1:H7]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: U2FRJ0