Detailed information of TA system TA231663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2662292..2662912
• Replicon: chromosome
• Accession: NZ_CP090698
• Organism: Burkholderia cepacia strain 2017Y70952565VI

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Z01_RS11725
• Protein ID: WP_021157289.1
• Coordinates: 2662292..2662609 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: L0Z01_RS11730
• Protein ID: WP_021157290.1
• Coordinates: 2662613..2662912 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z01_RS11715 (L0Z01_11715)	2658828..2660261	-	1434	WP_021157287.1	aldehyde dehydrogenase family protein	-
L0Z01_RS11720 (L0Z01_11720)	2660291..2661931	-	1641	WP_021157288.1	acetolactate synthase large subunit	-
L0Z01_RS11725 (L0Z01_11725)	2662292..2662609	+	318	WP_021157289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z01_RS11730 (L0Z01_11730)	2662613..2662912	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
L0Z01_RS11735 (L0Z01_11735)	2662969..2664255	-	1287	WP_060343380.1	DUF445 domain-containing protein	-
L0Z01_RS11740 (L0Z01_11740)	2664467..2665687	-	1221	WP_249453804.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L0Z01_RS11745 (L0Z01_11745)	2665677..2666003	-	327	WP_048022186.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L0Z01_RS11750 (L0Z01_11750)	2666024..2666509	-	486	WP_048022184.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L0Z01_RS11755 (L0Z01_11755)	2666527..2667798	-	1272	WP_048022287.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11866.62 Da
• Isoelectric Point: 9.9423

>T231663 WP_021157289.1 NZ_CP090698:2662292-2662609 [Burkholderia cepacia]
MPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T231663 NZ_CP126941:19957-20373 [Escherichia coli O2:K1:H7]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT231663 WP_021157290.1 NZ_CP090698:2662613-2662912 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT231663 NZ_CP126941:19730-19960 [Escherichia coli O2:K1:H7]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3