Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4462529..4463160 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ECH74115_RS23995 Protein ID WP_001259385.1
Coordinates 4462885..4463160 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECH74115_RS23990 Protein ID WP_000593555.1
Coordinates 4462529..4462888 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS23960 4457641..4458114 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECH74115_RS23965 4458111..4458392 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ECH74115_RS23970 4458469..4459827 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ECH74115_RS23975 4459820..4461328 + 1509 WP_001301773.1 carbohydrate kinase -
ECH74115_RS23980 4461318..4461587 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ECH74115_RS23985 4461619..4462479 + 861 WP_001131788.1 class II aldolase -
ECH74115_RS23990 4462529..4462888 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECH74115_RS23995 4462885..4463160 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ECH74115_RS24000 4463233..4464357 - 1125 WP_001301659.1 ABC transporter permease -
ECH74115_RS24005 4464357..4467092 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
ECH74115_RS24010 4467089..4468156 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T23166 WP_001259385.1 NC_011353:c4463160-4462885 [Escherichia coli O157:H7 str. EC4115]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T23166 NC_011353:c4463160-4462885 [Escherichia coli O157:H7 str. EC4115]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT23166 WP_000593555.1 NC_011353:c4462888-4462529 [Escherichia coli O157:H7 str. EC4115]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT23166 NC_011353:c4462888-4462529 [Escherichia coli O157:H7 str. EC4115]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References