Detailed information of TA system TA231658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1210988..1212738
• Replicon: chromosome
• Accession: NZ_CP090698
• Organism: Burkholderia cepacia strain 2017Y70952565VI

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: L0Z01_RS05405
• Protein ID: WP_249455088.1
• Coordinates: 1210988..1211884 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A365QHE5
• Locus tag: L0Z01_RS05410
• Protein ID: WP_059497041.1
• Coordinates: 1211881..1212738 (+)
• Length: 286 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z01_RS05370 (L0Z01_05370)	1206349..1207428	-	1080	WP_042976372.1	porin	-
L0Z01_RS05375 (L0Z01_05375)	1207453..1207779	+	327	WP_249455086.1	hypothetical protein	-
L0Z01_RS05380 (L0Z01_05380)	1207874..1208191	+	318	WP_021161517.1	non-heme iron oxygenase ferredoxin subunit	-
L0Z01_RS05385 (L0Z01_05385)	1208195..1208506	+	312	WP_059484801.1	hypothetical protein	-
L0Z01_RS05390 (L0Z01_05390)	1208575..1209645	+	1071	WP_021161515.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L0Z01_RS05395 (L0Z01_05395)	1209638..1210408	+	771	WP_249481299.1	SDR family oxidoreductase	-
L0Z01_RS05400 (L0Z01_05400)	1210442..1210969	+	528	WP_048022636.1	cupin domain-containing protein	-
L0Z01_RS05405 (L0Z01_05405)	1210988..1211884	+	897	WP_249455088.1	alpha/beta fold hydrolase	Toxin
L0Z01_RS05410 (L0Z01_05410)	1211881..1212738	+	858	WP_059497041.1	IclR family transcriptional regulator	Antitoxin
L0Z01_RS05415 (L0Z01_05415)	1212735..1213532	+	798	WP_059484804.1	SDR family oxidoreductase	-
L0Z01_RS05420 (L0Z01_05420)	1213525..1215192	+	1668	WP_021161509.1	thiamine pyrophosphate-binding protein	-
L0Z01_RS05425 (L0Z01_05425)	1215185..1216012	+	828	WP_021161508.1	aspartate dehydrogenase	-
L0Z01_RS05430 (L0Z01_05430)	1216085..1217575	+	1491	WP_059484807.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-279)

Antitoxin

• Transcription regulator IclR, C-terminal (143-266)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30584.78 Da
• Isoelectric Point: 7.7509

>T231658 WP_249455088.1 NZ_CP090698:1210988-1211884 [Burkholderia cepacia]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAAGTIGYREAGAQHAGRALPVVLLHGIGSGAASWVSQLDTLGASRRVLAW
DAPGYGVSTPVHGASPAAADYAASLNAWLEALGIERCVLVGHSLGAIVAGGLARVMPARIAGLLLVSPAGGYGSAPAETR
ESRRDARLAMLAELGPAGLAEQRSGNMLSGFANEEARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAHVGLQVIPQAGHAGYVEAPAVYSALIDSFCRHCDQQRGLA

Nucleotide

• Length: 897 bp

>T231658 NZ_CP126940:4220297-4220449 [Escherichia coli O2:K1:H7]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 286 a.a.
• Molecular weight: 31546.09 Da
• Isoelectric Point: 6.6572

>AT231658 WP_059497041.1 NZ_CP090698:1211881-1212738 [Burkholderia cepacia]
MNEPLQRPEPENDKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYK
LGIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSQSPVFSSIRVNVGTRLPAHATTHGH
VLMGDLSLKELRSLYPEGTLNRMTNATPETVDALYEVIREDALRGYGVSNSSFERGISVVTAPVRNETQKIVACITVTVP
RPEIDAALIADGLIDKVQRAAAELSRRLNYRSDDEHTFMKALGLR

Nucleotide

• Length: 858 bp

>AT231658 NZ_CP126940:c4220248-4220191 [Escherichia coli O2:K1:H7]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin

• AlphaFold DB: A0A365QHE5