Detailed information of TA system TA231655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 348283..348848
• Replicon: chromosome
• Accession: NZ_CP090698
• Organism: Burkholderia cepacia strain 2017Y70952565VI

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E1S7L3
• Locus tag: L0Z01_RS01620
• Protein ID: WP_060343311.1
• Coordinates: 348283..348654 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: L0Z01_RS01625
• Protein ID: WP_006752328.1
• Coordinates: 348651..348848 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z01_RS01605 (L0Z01_01605)	344146..344871	+	726	WP_021159298.1	site-2 protease family protein	-
L0Z01_RS01610 (L0Z01_01610)	344934..346313	-	1380	WP_059591042.1	MFS transporter	-
L0Z01_RS01615 (L0Z01_01615)	346559..348136	+	1578	WP_249454468.1	aldehyde dehydrogenase (NADP(+))	-
L0Z01_RS01620 (L0Z01_01620)	348283..348654	-	372	WP_060343311.1	PIN domain-containing protein	Toxin
L0Z01_RS01625 (L0Z01_01625)	348651..348848	-	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z01_RS01630 (L0Z01_01630)	349097..349648	+	552	WP_048023383.1	hypothetical protein	-
L0Z01_RS01635 (L0Z01_01635)	349832..350746	-	915	WP_048023382.1	5-dehydro-4-deoxyglucarate dehydratase	-
L0Z01_RS01640 (L0Z01_01640)	350975..352528	+	1554	WP_249454469.1	galactarate dehydratase	-
L0Z01_RS01645 (L0Z01_01645)	352595..353077	-	483	WP_042974910.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13384.42 Da
• Isoelectric Point: 5.5223

>T231655 WP_060343311.1 NZ_CP090698:c348654-348283 [Burkholderia cepacia]
MILVDTSVWIDHLRAGDATLTTLLEAERVLIHPFVVGELALGSLRDRETVLDALRDLPAAAAATDDEVQRMIDVAPLYGL
GIGYVDAHLLASVRLTSGSKLWTRDRRLLAAAERLQLAAHPAH

Nucleotide

• Length: 372 bp

>T231655 NZ_CP126940:c3710310-3710008 [Escherichia coli O2:K1:H7]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7054.10 Da
• Isoelectric Point: 10.6826

>AT231655 WP_006752328.1 NZ_CP090698:c348848-348651 [Burkholderia cepacia]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT231655 NZ_CP126940:c3709972-3709631 [Escherichia coli O2:K1:H7]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8