Detailed information of TA system TA231643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 875888..877635
• Replicon: chromosome
• Accession: NZ_CP090694
• Organism: Burkholderia multivorans strain 2017Y71079786

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: L0Y98_RS23790
• Protein ID: WP_048804598.1
• Coordinates: 875888..876784 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: L0Y98_RS23795
• Protein ID: WP_006396780.1
• Coordinates: 876781..877635 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y98_RS23760 (L0Y98_23760)	871288..872367	-	1080	WP_039217461.1	porin	-
L0Y98_RS23765 (L0Y98_23765)	872803..873120	+	318	WP_155825139.1	non-heme iron oxygenase ferredoxin subunit	-
L0Y98_RS23770 (L0Y98_23770)	873122..873433	+	312	WP_006396775.1	hypothetical protein	-
L0Y98_RS23775 (L0Y98_23775)	873481..874551	+	1071	WP_155825137.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L0Y98_RS23780 (L0Y98_23780)	874544..875314	+	771	WP_006413827.1	SDR family oxidoreductase	-
L0Y98_RS23785 (L0Y98_23785)	875342..875869	+	528	WP_006396778.1	cupin domain-containing protein	-
L0Y98_RS23790 (L0Y98_23790)	875888..876784	+	897	WP_048804598.1	alpha/beta fold hydrolase	Toxin
L0Y98_RS23795 (L0Y98_23795)	876781..877635	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
L0Y98_RS23800 (L0Y98_23800)	877632..878429	+	798	WP_155825136.1	SDR family oxidoreductase	-
L0Y98_RS23805 (L0Y98_23805)	878422..880089	+	1668	WP_155825135.1	thiamine pyrophosphate-binding protein	-
L0Y98_RS23810 (L0Y98_23810)	880082..880912	+	831	WP_006396783.1	aspartate dehydrogenase	-
L0Y98_RS23815 (L0Y98_23815)	880974..882464	+	1491	WP_155825134.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30583.83 Da
• Isoelectric Point: 8.0534

>T231643 WP_048804598.1 NZ_CP090694:875888-876784 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T231643 NZ_CP126940:196498-196605 [Escherichia coli O2:K1:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCACGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT231643 WP_006396780.1 NZ_CP090694:876781-877635 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT231643 NZ_CP126940:c196450-196384 [Escherichia coli O2:K1:H7]
GGTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: B9AZJ2