Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 150402..151048 Replicon plasmid p1_2017Y71087112IIIx
Accession NZ_CP090691
Organism Burkholderia multivorans strain 2017Y71087112III

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag L0Y89_RS30600 Protein ID WP_012211060.1
Coordinates 150641..151048 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag L0Y89_RS30595 Protein ID WP_009692911.1
Coordinates 150402..150644 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Y89_RS30575 (L0Y89_30575) 145537..146394 - 858 WP_012211056.1 plasmid replication initiator TrfA -
L0Y89_RS30580 (L0Y89_30580) 147139..148104 - 966 WP_012211057.1 ParB/RepB/Spo0J family partition protein -
L0Y89_RS30585 (L0Y89_30585) 148104..148775 - 672 WP_012211058.1 AAA family ATPase -
L0Y89_RS30590 (L0Y89_30590) 149332..150300 + 969 WP_231856867.1 non-homologous end-joining DNA ligase -
L0Y89_RS30595 (L0Y89_30595) 150402..150644 + 243 WP_009692911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
L0Y89_RS30600 (L0Y89_30600) 150641..151048 + 408 WP_012211060.1 type II toxin-antitoxin system VapC family toxin Toxin
L0Y89_RS30605 (L0Y89_30605) 151392..151712 + 321 WP_012211061.1 hypothetical protein -
L0Y89_RS30610 (L0Y89_30610) 151744..152352 + 609 WP_012211062.1 hypothetical protein -
L0Y89_RS30615 (L0Y89_30615) 152349..152507 + 159 WP_009692916.1 hypothetical protein -
L0Y89_RS30620 (L0Y89_30620) 152500..153420 + 921 WP_009692917.1 serine protease -
L0Y89_RS30625 (L0Y89_30625) 153532..153717 + 186 WP_009692918.1 hypothetical protein -
L0Y89_RS30630 (L0Y89_30630) 153728..154036 + 309 WP_009692919.1 hypothetical protein -
L0Y89_RS30635 (L0Y89_30635) 154033..154764 + 732 WP_009692920.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..156152 156152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(12-49)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14613.77 Da        Isoelectric Point: 5.1402

>T231637 WP_012211060.1 NZ_CP090691:150641-151048 [Burkholderia multivorans]
MSLYLLDTNILSNVIRDPRGACASRIGETRPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHAMAVDAVLVTDNTAEFTRVTGLAVENWLRPA

Download         Length: 408 bp

>T231637 NZ_CP126937:4110807-4111148 [Escherichia coli O2:K1:H4]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9309.54 Da        Isoelectric Point: 4.8615

>AT231637 WP_009692911.1 NZ_CP090691:150402-150644 [Burkholderia multivorans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Download         Length: 243 bp

>AT231637 NZ_CP126937:c4110812-4110736 [Escherichia coli O2:K1:H4]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References