Detailed information of TA system TA231632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1494756..1496503
• Replicon: chromosome
• Accession: NZ_CP090688
• Organism: Burkholderia multivorans strain 2017Y71087112III

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: L0Y89_RS06595
• Protein ID: WP_105836409.1
• Coordinates: 1495607..1496503 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: L0Y89_RS06590
• Protein ID: WP_006396780.1
• Coordinates: 1494756..1495610 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y89_RS06570 (L0Y89_06570)	1489931..1491421	-	1491	WP_043278809.1	aldehyde dehydrogenase	-
L0Y89_RS06575 (L0Y89_06575)	1491479..1492309	-	831	WP_105836412.1	aspartate dehydrogenase	-
L0Y89_RS06580 (L0Y89_06580)	1492302..1493969	-	1668	WP_249458835.1	thiamine pyrophosphate-binding protein	-
L0Y89_RS06585 (L0Y89_06585)	1493962..1494759	-	798	WP_006396781.1	SDR family oxidoreductase	-
L0Y89_RS06590 (L0Y89_06590)	1494756..1495610	-	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
L0Y89_RS06595 (L0Y89_06595)	1495607..1496503	-	897	WP_105836409.1	alpha/beta fold hydrolase	Toxin
L0Y89_RS06600 (L0Y89_06600)	1496522..1497049	-	528	WP_006396778.1	cupin domain-containing protein	-
L0Y89_RS06605 (L0Y89_06605)	1497077..1497847	-	771	WP_006413827.1	SDR family oxidoreductase	-
L0Y89_RS06610 (L0Y89_06610)	1497840..1498910	-	1071	WP_175848021.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L0Y89_RS06615 (L0Y89_06615)	1498958..1499269	-	312	WP_006396775.1	hypothetical protein	-
L0Y89_RS06620 (L0Y89_06620)	1499271..1499588	-	318	WP_006396774.1	non-heme iron oxygenase ferredoxin subunit	-
L0Y89_RS06625 (L0Y89_06625)	1500024..1501103	+	1080	WP_039217461.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30543.77 Da
• Isoelectric Point: 8.0534

>T231632 WP_105836409.1 NZ_CP090688:c1496503-1495607 [Burkholderia multivorans]
MSVVDKRERDRTAAFGTLLERFPEQRCAAGAAGTIGYREAGAQHAGAALAVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T231632 NZ_CP126937:2781846-2781953 [Escherichia coli O2:K1:H4]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT231632 WP_006396780.1 NZ_CP090688:c1495610-1494756 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT231632 NZ_CP126937:c2781793-2781732 [Escherichia coli O2:K1:H4]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: B9AZJ2