Detailed information of TA system TA231629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1045994..1047033
• Replicon: chromosome
• Accession: NZ_CP090688
• Organism: Burkholderia multivorans strain 2017Y71087112III

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A1W0YRD2
• Locus tag: L0Y89_RS04705
• Protein ID: WP_060096181.1
• Coordinates: 1046458..1047033 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: L0Y89_RS04700
• Protein ID: WP_054316743.1
• Coordinates: 1045994..1046461 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y89_RS04675 (L0Y89_04675)	1041464..1042927	+	1464	WP_105836663.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-
L0Y89_RS04680 (L0Y89_04680)	1042970..1043818	+	849	WP_012217098.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
L0Y89_RS04685 (L0Y89_04685)	1043854..1044246	+	393	WP_006403681.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
L0Y89_RS04690 (L0Y89_04690)	1044261..1045064	+	804	WP_105842135.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
L0Y89_RS04695 (L0Y89_04695)	1045072..1045878	+	807	WP_105836661.1	4-hydroxy-2-oxoheptanedioate aldolase	-
L0Y89_RS04700 (L0Y89_04700)	1045994..1046461	+	468	WP_054316743.1	helix-turn-helix domain-containing protein	Antitoxin
L0Y89_RS04705 (L0Y89_04705)	1046458..1047033	+	576	WP_060096181.1	PIN domain-containing protein	Toxin
L0Y89_RS04710 (L0Y89_04710)	1047278..1048087	-	810	WP_105836660.1	helix-turn-helix transcriptional regulator	-
L0Y89_RS04715 (L0Y89_04715)	1048183..1048434	+	252	WP_039218251.1	hypothetical protein	-
L0Y89_RS04720 (L0Y89_04720)	1048513..1049478	+	966	WP_105836659.1	zinc-binding alcohol dehydrogenase family protein	-
L0Y89_RS04725 (L0Y89_04725)	1049537..1049647	-	111	Protein_942	porin	-
L0Y89_RS04730 (L0Y89_04730)	1049775..1050425	+	651	WP_244130927.1	hypothetical protein	-
L0Y89_RS04735 (L0Y89_04735)	1050561..1051643	-	1083	WP_006415045.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21554.67 Da
• Isoelectric Point: 5.0551

>T231629 WP_060096181.1 NZ_CP090688:1046458-1047033 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDNLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T231629 NZ_CP126937:2172273-2172375 [Escherichia coli O2:K1:H4]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 6.1443

>AT231629 WP_054316743.1 NZ_CP090688:1045994-1046461 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEGQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELGKQAQALRMGYE

Nucleotide

• Length: 468 bp

>AT231629 NZ_CP126937:c2172413-2172145 [Escherichia coli O2:K1:H4]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A1W0YRD2