Detailed information of TA system TA231626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1369197..1369778
• Replicon: chromosome
• Accession: NZ_CP090687
• Organism: Burkholderia multivorans strain 2019Y60005016V

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Y84_RS19030
• Protein ID: WP_035946056.1
• Coordinates: 1369485..1369778 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: L0Y84_RS19025
• Protein ID: WP_006409266.1
• Coordinates: 1369197..1369481 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y84_RS19000 (L0Y84_19000)	1364845..1366266	-	1422	WP_043279886.1	FAD-binding oxidoreductase	-
L0Y84_RS19005 (L0Y84_19005)	1366285..1366680	-	396	WP_006404970.1	DUF2069 domain-containing protein	-
L0Y84_RS19010 (L0Y84_19010)	1366677..1367288	-	612	WP_006402099.1	NAD(P)H:quinone oxidoreductase	-
L0Y84_RS19015 (L0Y84_19015)	1367433..1368761	+	1329	WP_045594112.1	YihY family inner membrane protein	-
L0Y84_RS19020 (L0Y84_19020)	1368925..1369113	-	189	WP_006409259.1	hypothetical protein	-
L0Y84_RS19025 (L0Y84_19025)	1369197..1369481	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
L0Y84_RS19030 (L0Y84_19030)	1369485..1369778	-	294	WP_035946056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Y84_RS19035 (L0Y84_19035)	1370004..1370321	-	318	WP_006402097.1	DUF962 domain-containing protein	-
L0Y84_RS19040 (L0Y84_19040)	1370482..1370937	+	456	WP_006402096.1	CBS domain-containing protein	-
L0Y84_RS19045 (L0Y84_19045)	1371086..1373020	+	1935	WP_006404968.1	MFS transporter	-
L0Y84_RS19050 (L0Y84_19050)	1373189..1374289	+	1101	WP_006404967.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-88)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11168.92 Da
• Isoelectric Point: 9.4615

>T231626 WP_035946056.1 NZ_CP090687:c1369778-1369485 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKPVQMARELKE

Nucleotide

• Length: 294 bp

>T231626 NZ_CP126937:1983787-1984038 [Escherichia coli O2:K1:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT231626 WP_006409266.1 NZ_CP090687:c1369481-1369197 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT231626 NZ_CP126937:1984035-1984289 [Escherichia coli O2:K1:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: J4SGI3