Detailed information of TA system TA231625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 77437..78074
• Replicon: chromosome
• Accession: NZ_CP090687
• Organism: Burkholderia multivorans strain 2019Y60005016V

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Y84_RS12955
• Protein ID: WP_035489546.1
• Coordinates: 77673..78074 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Y84_RS12950
• Protein ID: WP_006408845.1
• Coordinates: 77437..77673 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y84_RS12930 (L0Y84_12930)	72598..73140	+	543	WP_006408276.1	hypothetical protein	-
L0Y84_RS12935 (L0Y84_12935)	73311..74342	+	1032	WP_045594318.1	helix-turn-helix domain-containing protein	-
L0Y84_RS12940 (L0Y84_12940)	74461..75549	-	1089	WP_006408279.1	hypothetical protein	-
L0Y84_RS12945 (L0Y84_12945)	75546..77249	-	1704	WP_006408280.1	thiamine pyrophosphate-binding protein	-
L0Y84_RS12950 (L0Y84_12950)	77437..77673	+	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Y84_RS12955 (L0Y84_12955)	77673..78074	+	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Y84_RS12960 (L0Y84_12960)	78196..79584	-	1389	WP_006408285.1	L-serine ammonia-lyase	-
L0Y84_RS12965 (L0Y84_12965)	79611..80753	-	1143	WP_006408286.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231625 WP_035489546.1 NZ_CP090687:77673-78074 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231625 NZ_CP126937:1191399-1191734 [Escherichia coli O2:K1:H4]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231625 WP_006408845.1 NZ_CP090687:77437-77673 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231625 NZ_CP126937:1191151-1191399 [Escherichia coli O2:K1:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1