Detailed information of TA system TA231621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 939350..941097
• Replicon: chromosome
• Accession: NZ_CP090686
• Organism: Burkholderia multivorans strain 2019Y60005016V

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B9BHC0
• Locus tag: L0Y84_RS06375
• Protein ID: WP_006403210.1
• Coordinates: 939350..940246 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: L0Y84_RS06380
• Protein ID: WP_006396780.1
• Coordinates: 940243..941097 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y84_RS06345 (L0Y84_06345)	934749..935828	-	1080	WP_006409825.1	porin	-
L0Y84_RS06350 (L0Y84_06350)	936265..936582	+	318	WP_006396774.1	non-heme iron oxygenase ferredoxin subunit	-
L0Y84_RS06355 (L0Y84_06355)	936584..936895	+	312	WP_006403207.1	hypothetical protein	-
L0Y84_RS06360 (L0Y84_06360)	936943..938013	+	1071	WP_006403208.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L0Y84_RS06365 (L0Y84_06365)	938006..938776	+	771	WP_006408452.1	SDR family oxidoreductase	-
L0Y84_RS06370 (L0Y84_06370)	938804..939331	+	528	WP_006396778.1	cupin domain-containing protein	-
L0Y84_RS06375 (L0Y84_06375)	939350..940246	+	897	WP_006403210.1	alpha/beta fold hydrolase	Toxin
L0Y84_RS06380 (L0Y84_06380)	940243..941097	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
L0Y84_RS06385 (L0Y84_06385)	941094..941891	+	798	WP_006409174.1	SDR family oxidoreductase	-
L0Y84_RS06390 (L0Y84_06390)	941884..943551	+	1668	WP_006403211.1	thiamine pyrophosphate-binding protein	-
L0Y84_RS06395 (L0Y84_06395)	943544..944374	+	831	WP_006403212.1	aspartate dehydrogenase	-
L0Y84_RS06400 (L0Y84_06400)	944436..945926	+	1491	WP_006403213.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30544.80 Da
• Isoelectric Point: 8.0446

>T231621 WP_006403210.1 NZ_CP090686:939350-940246 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQQAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDAFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T231621 NZ_CP126937:216078-216185 [Escherichia coli O2:K1:H4]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT231621 WP_006396780.1 NZ_CP090686:940243-941097 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT231621 NZ_CP126937:c216021-215964 [Escherichia coli O2:K1:H4]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: B9BHC0

Antitoxin

• AlphaFold DB: B9AZJ2