Detailed information of TA system TA231618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 131489..132135
• Replicon: plasmid p1_2019Y71417852IX
• Accession: NZ_CP090684
• Organism: Burkholderia multivorans strain 2019Y71417852IX

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z03_RS30505
• Protein ID: WP_012211060.1
• Coordinates: 131489..131896 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z03_RS30510
• Protein ID: WP_009692911.1
• Coordinates: 131893..132135 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z03_RS30470 (L0Z03_30470)	127773..128504	-	732	WP_009692920.1	hypothetical protein	-
L0Z03_RS30475 (L0Z03_30475)	128501..128809	-	309	WP_009692919.1	hypothetical protein	-
L0Z03_RS30480 (L0Z03_30480)	128820..129005	-	186	WP_009692918.1	hypothetical protein	-
L0Z03_RS30485 (L0Z03_30485)	129117..130037	-	921	WP_009692917.1	serine protease	-
L0Z03_RS30490 (L0Z03_30490)	130030..130188	-	159	WP_009692916.1	hypothetical protein	-
L0Z03_RS30495 (L0Z03_30495)	130185..130793	-	609	WP_012211062.1	hypothetical protein	-
L0Z03_RS30500 (L0Z03_30500)	130825..131145	-	321	WP_012211061.1	hypothetical protein	-
L0Z03_RS30505 (L0Z03_30505)	131489..131896	-	408	WP_012211060.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z03_RS30510 (L0Z03_30510)	131893..132135	-	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Z03_RS30515 (L0Z03_30515)	132237..133205	-	969	WP_231856867.1	non-homologous end-joining DNA ligase	-
L0Z03_RS30520 (L0Z03_30520)	133762..134433	+	672	WP_012211058.1	AAA family ATPase	-
L0Z03_RS30525 (L0Z03_30525)	134433..135398	+	966	WP_012211057.1	ParB/RepB/Spo0J family partition protein	-
L0Z03_RS30530 (L0Z03_30530)	136143..137000	+	858	WP_012211056.1	plasmid replication initiator TrfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..156151	156151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14613.77 Da
• Isoelectric Point: 5.1402

>T231618 WP_012211060.1 NZ_CP090684:c131896-131489 [Burkholderia multivorans]
MSLYLLDTNILSNVIRDPRGACASRIGETRPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHAMAVDAVLVTDNTAEFTRVTGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T231618 NZ_CP126937:c44229-43852 [Escherichia coli O2:K1:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT231618 WP_009692911.1 NZ_CP090684:c132135-131893 [Burkholderia multivorans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT231618 NZ_CP126937:c44653-44276 [Escherichia coli O2:K1:H4]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA