Detailed information of TA system TA231606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 77349..77986
• Replicon: chromosome
• Accession: NZ_CP090680
• Organism: Burkholderia multivorans strain 2019Y71421880II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z16_RS13595
• Protein ID: WP_065494376.1
• Coordinates: 77585..77986 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z16_RS13590
• Protein ID: WP_006408845.1
• Coordinates: 77349..77585 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z16_RS13570 (L0Z16_13570)	72501..73043	+	543	WP_053296681.1	hypothetical protein	-
L0Z16_RS13575 (L0Z16_13575)	73214..74245	+	1032	WP_012212492.1	helix-turn-helix domain-containing protein	-
L0Z16_RS13580 (L0Z16_13580)	74364..75461	-	1098	WP_065494378.1	ADP-heptose--LPS heptosyltransferase	-
L0Z16_RS13585 (L0Z16_13585)	75458..77161	-	1704	WP_065494377.1	thiamine pyrophosphate-binding protein	-
L0Z16_RS13590 (L0Z16_13590)	77349..77585	+	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z16_RS13595 (L0Z16_13595)	77585..77986	+	402	WP_065494376.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z16_RS13600 (L0Z16_13600)	78108..79496	-	1389	WP_065494375.1	L-serine ammonia-lyase	-
L0Z16_RS13605 (L0Z16_13605)	79523..80665	-	1143	WP_053296686.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15048.28 Da
• Isoelectric Point: 6.4573

>T231606 WP_065494376.1 NZ_CP090680:77585-77986 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAVTYAELEYGVTLCANPARERRNLAALIEDIPVAPFDGAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPRLRLENWLIG

Nucleotide

• Length: 402 bp

>T231606 NZ_CP126934:c2707162-2706755 [Escherichia coli O78:H4]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231606 WP_006408845.1 NZ_CP090680:77349-77585 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231606 NZ_CP126934:c2707409-2707143 [Escherichia coli O78:H4]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1