Detailed information of TA system TA231602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 968889..969466
• Replicon: chromosome
• Accession: NZ_CP090678
• Organism: Burkholderia multivorans strain 2019Y71421880II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z16_RS04290
• Protein ID: WP_053298683.1
• Coordinates: 968889..969254 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z16_RS04295
• Protein ID: WP_035956044.1
• Coordinates: 969248..969466 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z16_RS04260 (L0Z16_04260)	964066..964965	+	900	WP_249485433.1	lipid A hydroxylase LpxO	-
L0Z16_RS04265 (L0Z16_04265)	965089..965817	+	729	WP_249485435.1	hypothetical protein	-
L0Z16_RS04270 (L0Z16_04270)	965879..967477	-	1599	WP_249485438.1	alkyl hydroperoxide reductase subunit F	-
L0Z16_RS04275 (L0Z16_04275)	967596..968159	-	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z16_RS04280 (L0Z16_04280)	968408..968566	+	159	WP_200053153.1	hypothetical protein	-
L0Z16_RS04285 (L0Z16_04285)	968605..968793	-	189	WP_200053152.1	hypothetical protein	-
L0Z16_RS04290 (L0Z16_04290)	968889..969254	-	366	WP_053298683.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z16_RS04295 (L0Z16_04295)	969248..969466	-	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z16_RS04300 (L0Z16_04300)	969568..970464	-	897	WP_035954582.1	LysR substrate-binding domain-containing protein	-
L0Z16_RS04305 (L0Z16_04305)	970640..971806	+	1167	WP_059936587.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z16_RS04310 (L0Z16_04310)	971926..972465	+	540	WP_006403831.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z16_RS04315 (L0Z16_04315)	972458..973729	+	1272	WP_249485440.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13556.55 Da
• Isoelectric Point: 5.1515

>T231602 WP_053298683.1 NZ_CP090678:c969254-968889 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231602 NZ_CP126934:c1725230-1724979 [Escherichia coli O78:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231602 WP_035956044.1 NZ_CP090678:c969466-969248 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231602 NZ_CP126934:c1724982-1724728 [Escherichia coli O78:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45