Detailed information of TA system TA231600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 609119..610389
• Replicon: chromosome
• Accession: NZ_CP090678
• Organism: Burkholderia multivorans strain 2019Y71421880II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z16_RS02585
• Protein ID: WP_105774903.1
• Coordinates: 609119..609697 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9BS12
• Locus tag: L0Z16_RS02590
• Protein ID: WP_006406126.1
• Coordinates: 609832..610389 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z16_RS02565 (L0Z16_02565)	604406..605197	+	792	WP_035947285.1	enoyl-CoA hydratase	-
L0Z16_RS02570 (L0Z16_02570)	605194..606333	+	1140	WP_105774901.1	enoyl-CoA hydratase/isomerase family protein	-
L0Z16_RS02575 (L0Z16_02575)	606380..607300	-	921	WP_065496151.1	LysR family transcriptional regulator	-
L0Z16_RS02580 (L0Z16_02580)	607480..609018	+	1539	WP_105774902.1	MFS transporter	-
L0Z16_RS02585 (L0Z16_02585)	609119..609697	-	579	WP_105774903.1	HD domain-containing protein	Toxin
L0Z16_RS02590 (L0Z16_02590)	609832..610389	-	558	WP_006406126.1	helix-turn-helix transcriptional regulator	Antitoxin
L0Z16_RS02595 (L0Z16_02595)	610902..612305	+	1404	WP_053298294.1	hypothetical protein	-
L0Z16_RS02600 (L0Z16_02600)	612316..612936	-	621	WP_217063826.1	isochorismatase family cysteine hydrolase	-
L0Z16_RS02605 (L0Z16_02605)	613525..613968	-	444	WP_223852633.1	antibiotic biosynthesis monooxygenase	-
L0Z16_RS02610 (L0Z16_02610)	614068..615093	-	1026	WP_006406131.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21461.19 Da
• Isoelectric Point: 5.2986

>T231600 WP_105774903.1 NZ_CP090678:c609697-609119 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRRESTAEHSWRLCLMALVFADALHGIDTLKLLKLCVVHDLGEALHGDIP
APEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDVAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAHT

Nucleotide

• Length: 579 bp

>T231600 NZ_CP126934:1713086-1713460 [Escherichia coli O78:H4]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTCAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20378.30 Da
• Isoelectric Point: 7.8341

>AT231600 WP_006406126.1 NZ_CP090678:c610389-609832 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAAHLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMETALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT231600 NZ_CP126934:1712629-1712997 [Escherichia coli O78:H4]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: B9BS12