Detailed information of TA system TA231594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 734325..734857
• Replicon: chromosome
• Accession: NZ_CP090676
• Organism: Burkholderia cenocepacia strain 2019Y71443953II

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: L0Y99_RS07890
• Protein ID: WP_006484901.1
• Coordinates: 734325..734648 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: L0Y99_RS07895
• Protein ID: WP_012492929.1
• Coordinates: 734645..734857 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y99_RS07860 (L0Y99_07860)	729336..730169	-	834	WP_034200961.1	class II glutamine amidotransferase	-
L0Y99_RS07865 (L0Y99_07865)	730369..730698	-	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
L0Y99_RS07870 (L0Y99_07870)	730828..731775	+	948	WP_205671098.1	AraC family transcriptional regulator	-
L0Y99_RS07875 (L0Y99_07875)	731879..732322	+	444	WP_011544671.1	inner membrane protein YiaA	-
L0Y99_RS07880 (L0Y99_07880)	732445..733356	-	912	WP_240465854.1	relaxase/mobilization nuclease domain-containing protein	-
L0Y99_RS07885 (L0Y99_07885)	733481..733798	-	318	WP_226245292.1	hypothetical protein	-
L0Y99_RS07890 (L0Y99_07890)	734325..734648	-	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0Y99_RS07895 (L0Y99_07895)	734645..734857	-	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
L0Y99_RS07900 (L0Y99_07900)	735005..735457	-	453	WP_006484947.1	hypothetical protein	-
L0Y99_RS07905 (L0Y99_07905)	735525..735920	-	396	WP_205671096.1	hypothetical protein	-
L0Y99_RS07910 (L0Y99_07910)	736059..736379	+	321	WP_012492928.1	helix-turn-helix transcriptional regulator	-
L0Y99_RS07920 (L0Y99_07920)	736806..737555	-	750	WP_006484965.1	tol-pal system protein YbgF	-
L0Y99_RS07925 (L0Y99_07925)	737575..738087	-	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
L0Y99_RS07930 (L0Y99_07930)	738105..739400	-	1296	WP_012492927.1	Tol-Pal system beta propeller repeat protein TolB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T231594 WP_006484901.1 NZ_CP090676:c734648-734325 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T231594 NZ_CP126933:c64394-64014 [Escherichia coli O78:H51]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT231594 WP_012492929.1 NZ_CP090676:c734857-734645 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT231594 NZ_CP126933:c64615-64394 [Escherichia coli O78:H51]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7