Detailed information of TA system TA231582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 171049..171637
• Replicon: plasmid pPA1609-475
• Accession: NZ_CP090650
• Organism: Pseudomonas aeruginosa strain PA1609

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1H7JCY8
• Locus tag: L0026_RS32830
• Protein ID: WP_074866086.1
• Coordinates: 171335..171637 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L0026_RS32825
• Protein ID: WP_247322191.1
• Coordinates: 171049..171342 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0026_RS32810 (L0026_32795)	166286..166699	-	414	WP_247322181.1	helix-turn-helix transcriptional regulator	-
L0026_RS32815 (L0026_32800)	166802..169891	+	3090	WP_247322184.1	CHC2 zinc finger domain-containing protein	-
L0026_RS32820 (L0026_32805)	169900..170985	+	1086	WP_247322188.1	site-specific tyrosine recombinase XerC	-
L0026_RS32825 (L0026_32810)	171049..171342	-	294	WP_247322191.1	putative addiction module antidote protein	Antitoxin
L0026_RS32830 (L0026_32815)	171335..171637	-	303	WP_074866086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0026_RS32835 (L0026_32820)	171943..172371	+	429	WP_260476597.1	cysteine peptidase family C39 domain-containing protein	-
L0026_RS32840 (L0026_32825)	172368..172724	+	357	WP_071535154.1	hypothetical protein	-
L0026_RS32845 (L0026_32830)	173167..173613	+	447	WP_247322175.1	hypothetical protein	-
L0026_RS32850 (L0026_32835)	173673..173918	-	246	WP_247322178.1	antitoxin VbhA family protein	-
L0026_RS32855 (L0026_32840)	174465..174878	-	414	WP_247322181.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrVC1 / ARR-3 / dfrA22 / qacE / sul1 / mph(E) / msr(E) / armA / blaOXA-1 / blaIMP-45	-	1..475129	475129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11293.95 Da
• Isoelectric Point: 10.1773

>T231582 WP_074866086.1 NZ_CP090650:c171637-171335 [Pseudomonas aeruginosa]
MKKIESSKFRTWLKKLKDDTGRARIVARINRLMEGLPGDVAPVGHGVSELRIHYGPGYRVYFHQQGDTLVILLCGGDKGS
QSRDIEAAHQILQAWRAQHD

Nucleotide

• Length: 303 bp

>T231582 NZ_CP126931:3698422-3698640 [Escherichia coli O78:H51]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10568.02 Da
• Isoelectric Point: 4.8129

>AT231582 WP_247322191.1 NZ_CP090650:c171342-171049 [Pseudomonas aeruginosa]
MTETFTDFDPAEYLTSPEAIAEFMSDALETGDAGYIAKAMGVVARARGMTELARETGLSREQLYRSFSEHGNPTLKTMLA
VMKALGVDMAARPHIAH

Nucleotide

• Length: 294 bp

>AT231582 NZ_CP126931:3698022-3698396 [Escherichia coli O78:H51]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1H7JCY8