Detailed information of TA system TA231576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1690517..1692212
• Replicon: chromosome
• Accession: NZ_CP090649
• Organism: Pseudomonas aeruginosa strain PA1609

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: L0026_RS07860
• Protein ID: WP_003112901.1
• Coordinates: 1690517..1691386 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: L0026_RS07865
• Protein ID: WP_003112902.1
• Coordinates: 1691379..1692212 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0026_RS07835 (L0026_07835)	1686525..1687376	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
L0026_RS07840 (L0026_07840)	1687417..1688424	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
L0026_RS07845 (L0026_07845)	1688421..1689197	+	777	WP_003112898.1	ABC transporter permease	-
L0026_RS07850 (L0026_07850)	1689203..1689964	+	762	WP_003112899.1	SDR family oxidoreductase	-
L0026_RS07855 (L0026_07855)	1689978..1690508	+	531	WP_003112900.1	cupin domain-containing protein	-
L0026_RS07860 (L0026_07860)	1690517..1691386	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
L0026_RS07865 (L0026_07865)	1691379..1692212	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
L0026_RS07870 (L0026_07870)	1692199..1692996	+	798	WP_003112903.1	SDR family oxidoreductase	-
L0026_RS07875 (L0026_07875)	1692989..1694671	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
L0026_RS07880 (L0026_07880)	1694682..1695485	+	804	WP_003130116.1	aspartate dehydrogenase	-
L0026_RS07885 (L0026_07885)	1695497..1696981	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T231576 WP_003112901.1 NZ_CP090649:1690517-1691386 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T231576 NZ_CP126931:1965513-1965767 [Escherichia coli O78:H51]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT231576 WP_003112902.1 NZ_CP090649:1691379-1692212 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT231576 NZ_CP126931:1965265-1965516 [Escherichia coli O78:H51]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1