Detailed information of TA system TA231575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 1107003..1107591
• Replicon: chromosome
• Accession: NZ_CP090649
• Organism: Pseudomonas aeruginosa strain PA1609

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1H7JCY8
• Locus tag: L0026_RS05215
• Protein ID: WP_074866086.1
• Coordinates: 1107289..1107591 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L0026_RS05210
• Protein ID: WP_247322191.1
• Coordinates: 1107003..1107296 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0026_RS05195 (L0026_05195)	1102240..1102653	-	414	WP_247322181.1	helix-turn-helix transcriptional regulator	-
L0026_RS05200 (L0026_05200)	1102756..1105845	+	3090	WP_247322184.1	CHC2 zinc finger domain-containing protein	-
L0026_RS05205 (L0026_05205)	1105854..1106939	+	1086	WP_247322188.1	site-specific tyrosine recombinase XerC	-
L0026_RS05210 (L0026_05210)	1107003..1107296	-	294	WP_247322191.1	putative addiction module antidote protein	Antitoxin
L0026_RS05215 (L0026_05215)	1107289..1107591	-	303	WP_074866086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0026_RS05220 (L0026_05220)	1107942..1108325	+	384	WP_235549841.1	cysteine peptidase family C39 domain-containing protein	-
L0026_RS05225 (L0026_05225)	1108322..1108678	+	357	WP_071535154.1	hypothetical protein	-
L0026_RS05230 (L0026_05230)	1109121..1109567	+	447	WP_247322175.1	hypothetical protein	-
L0026_RS05235 (L0026_05235)	1109627..1109872	-	246	WP_247322178.1	antitoxin VbhA family protein	-
L0026_RS05240 (L0026_05240)	1110419..1110832	-	414	WP_247322181.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11293.95 Da
• Isoelectric Point: 10.1773

>T231575 WP_074866086.1 NZ_CP090649:c1107591-1107289 [Pseudomonas aeruginosa]
MKKIESSKFRTWLKKLKDDTGRARIVARINRLMEGLPGDVAPVGHGVSELRIHYGPGYRVYFHQQGDTLVILLCGGDKGS
QSRDIEAAHQILQAWRAQHD

Nucleotide

• Length: 303 bp

>T231575 NZ_CP126931:1965265-1965516 [Escherichia coli O78:H51]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10568.02 Da
• Isoelectric Point: 4.8129

>AT231575 WP_247322191.1 NZ_CP090649:c1107296-1107003 [Pseudomonas aeruginosa]
MTETFTDFDPAEYLTSPEAIAEFMSDALETGDAGYIAKAMGVVARARGMTELARETGLSREQLYRSFSEHGNPTLKTMLA
VMKALGVDMAARPHIAH

Nucleotide

• Length: 294 bp

>AT231575 NZ_CP126931:1965513-1965767 [Escherichia coli O78:H51]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A1H7JCY8