Detailed information of TA system TA231568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 556357..557615
• Replicon: chromosome
• Accession: NZ_CP090648
• Organism: Pseudomonas aeruginosa strain PA1616

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: L0025_RS02630
• Protein ID: WP_003112528.1
• Coordinates: 556357..557112 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: L0025_RS02635
• Protein ID: WP_003086329.1
• Coordinates: 557247..557615 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0025_RS02620 (L0025_02620)	551530..554073	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
L0025_RS02625 (L0025_02625)	554228..555589	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
L0025_RS02630 (L0025_02630)	556357..557112	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
L0025_RS02635 (L0025_02635)	557247..557615	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
L0025_RS02640 (L0025_02640)	557688..558974	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
L0025_RS02645 (L0025_02645)	559030..560523	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
L0025_RS02650 (L0025_02650)	560698..561174	+	477	WP_003112531.1	hypothetical protein	-
L0025_RS02655 (L0025_02655)	561177..561452	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T231568 WP_003112528.1 NZ_CP090648:c557112-556357 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T231568 NZ_CP126931:313667-313897 [Escherichia coli O78:H51]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT231568 WP_003086329.1 NZ_CP090648:c557615-557247 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT231568 NZ_CP126931:313894-314253 [Escherichia coli O78:H51]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7