Detailed information of TA system TA231566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2637690..2638911
• Replicon: chromosome
• Accession: NZ_CP090647
• Organism: Pseudomonas aeruginosa strain PA1681

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: L0024_RS12370
• Protein ID: WP_003102740.1
• Coordinates: 2637690..2638268 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0024_RS12375
• Protein ID: WP_003088112.1
• Coordinates: 2638357..2638911 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0024_RS12355 (L0024_12355)	2632967..2634244	+	1278	WP_003102743.1	TolC family protein	-
L0024_RS12360 (L0024_12360)	2634234..2636405	+	2172	WP_034017575.1	type I secretion system permease/ATPase	-
L0024_RS12365 (L0024_12365)	2636395..2637582	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
L0024_RS12370 (L0024_12370)	2637690..2638268	-	579	WP_003102740.1	HD domain-containing protein	Toxin
L0024_RS12375 (L0024_12375)	2638357..2638911	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
L0024_RS12380 (L0024_12380)	2639069..2641264	-	2196	WP_034017576.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
L0024_RS12385 (L0024_12385)	2641269..2641730	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
L0024_RS12390 (L0024_12390)	2642061..2642384	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
L0024_RS12395 (L0024_12395)	2642397..2642795	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
L0024_RS12400 (L0024_12400)	2642792..2643343	-	552	WP_003450159.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T231566 WP_003102740.1 NZ_CP090647:c2638268-2637690 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T231566 NZ_CP126929:176699-177097 [Escherichia coli O2:K1:H4]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT231566 WP_003088112.1 NZ_CP090647:c2638911-2638357 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT231566 NZ_CP126929:176472-176699 [Escherichia coli O2:K1:H4]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07