Detailed information of TA system TA231563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 142074..142579
• Replicon: chromosome
• Accession: NZ_CP090647
• Organism: Pseudomonas aeruginosa strain PA1681

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: L0024_RS00655
• Protein ID: WP_003083773.1
• Coordinates: 142074..142355 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: L0024_RS00660
• Protein ID: WP_003083775.1
• Coordinates: 142352..142579 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0024_RS00630 (L0024_00630)	137325..138674	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
L0024_RS00635 (L0024_00635)	138723..139409	+	687	WP_033888123.1	FadR/GntR family transcriptional regulator	-
L0024_RS00640 (L0024_00640)	139510..140244	+	735	WP_003117208.1	GntR family transcriptional regulator	-
L0024_RS00645 (L0024_00645)	140424..140834	+	411	WP_003101225.1	aegerolysin family protein	-
L0024_RS00650 (L0024_00650)	140866..141774	-	909	WP_003083769.1	LysR family transcriptional regulator	-
L0024_RS00655 (L0024_00655)	142074..142355	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
L0024_RS00660 (L0024_00660)	142352..142579	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
L0024_RS00665 (L0024_00665)	142755..143375	-	621	WP_003101226.1	hypothetical protein	-
L0024_RS00670 (L0024_00670)	143476..143976	+	501	WP_003101228.1	LEA type 2 family protein	-
L0024_RS00675 (L0024_00675)	144049..144390	+	342	WP_034017210.1	zinc ribbon domain-containing protein YjdM	-
L0024_RS00680 (L0024_00680)	144471..145898	-	1428	WP_003083784.1	GABA permease	-
L0024_RS00685 (L0024_00685)	146067..147560	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T231563 WP_003083773.1 NZ_CP090647:c142355-142074 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T231563 NZ_CP126929:41976-42392 [Escherichia coli O2:K1:H4]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT231563 WP_003083775.1 NZ_CP090647:c142579-142352 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT231563 NZ_CP126929:41749-41979 [Escherichia coli O2:K1:H4]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9