Detailed information of TA system TA231561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 93132..93778
• Replicon: plasmid unnamed3
• Accession: NZ_CP090645
• Organism: Burkholderia contaminans strain LMG 23361

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LXE91_RS42790
• Protein ID: WP_009692912.1
• Coordinates: 93132..93539 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LXE91_RS42795
• Protein ID: WP_009692911.1
• Coordinates: 93536..93778 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXE91_RS42755 (LXE91_42665)	88913..89566	-	654	WP_226292009.1	hypothetical protein	-
LXE91_RS42760 (LXE91_42670)	89629..89937	-	309	WP_069301923.1	hypothetical protein	-
LXE91_RS42765 (LXE91_42675)	89954..90244	-	291	WP_069301924.1	hypothetical protein	-
LXE91_RS42770 (LXE91_42680)	90265..91164	-	900	WP_069301925.1	hypothetical protein	-
LXE91_RS42775 (LXE91_42685)	91161..91769	-	609	WP_226292010.1	hypothetical protein	-
LXE91_RS42780 (LXE91_42690)	91804..92115	-	312	WP_069301926.1	hypothetical protein	-
LXE91_RS42785 (LXE91_42695)	92459..92989	-	531	WP_141716966.1	hypothetical protein	-
LXE91_RS42790 (LXE91_42700)	93132..93539	-	408	WP_009692912.1	type II toxin-antitoxin system VapC family toxin	Toxin
LXE91_RS42795 (LXE91_42705)	93536..93778	-	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LXE91_RS42800	93765..94013	-	249	WP_252447792.1	hypothetical protein	-
LXE91_RS42805	94060..94386	-	327	WP_009692910.1	DNA polymerase LigD ligase subunit	-
LXE91_RS42810 (LXE91_42715)	94412..94654	-	243	WP_226292011.1	hypothetical protein	-
LXE91_RS42815 (LXE91_42720)	95567..96253	+	687	WP_009692909.1	ParA family partition ATPase	-
LXE91_RS42820 (LXE91_42725)	96250..97293	+	1044	WP_069301928.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..162811	162811

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14506.64 Da
• Isoelectric Point: 4.8230

>T231561 WP_009692912.1 NZ_CP090645:c93539-93132 [Burkholderia contaminans]
MSLYLLDTNILSNVIRDPRGACASRIGETPPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T231561 NZ_CP126929:c32703-32398 [Escherichia coli O2:K1:H4]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT231561 WP_009692911.1 NZ_CP090645:c93778-93536 [Burkholderia contaminans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT231561 NZ_CP126929:c32923-32705 [Escherichia coli O2:K1:H4]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGTACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA