Detailed information of TA system TA231559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 141687..142336
• Replicon: plasmid unnamed2
• Accession: NZ_CP090644
• Organism: Burkholderia contaminans strain LMG 23361

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LXE91_RS41870
• Protein ID: WP_027813527.1
• Coordinates: 141926..142336 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6P2T8K5
• Locus tag: LXE91_RS41865
• Protein ID: WP_027813526.1
• Coordinates: 141687..141929 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXE91_RS41845 (LXE91_41750)	136904..137761	-	858	WP_027813522.1	plasmid replication initiator TrfA	-
LXE91_RS41850 (LXE91_41755)	138504..139469	-	966	WP_027813523.1	ParB/RepB/Spo0J family partition protein	-
LXE91_RS41855 (LXE91_41760)	139469..140140	-	672	WP_027813524.1	AAA family ATPase	-
LXE91_RS41860 (LXE91_41765)	140606..141592	+	987	WP_223274426.1	non-homologous end-joining DNA ligase	-
LXE91_RS41865 (LXE91_41770)	141687..141929	+	243	WP_027813526.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LXE91_RS41870 (LXE91_41775)	141926..142336	+	411	WP_027813527.1	type II toxin-antitoxin system VapC family toxin	Toxin
LXE91_RS41875 (LXE91_41780)	142636..142995	+	360	WP_223274427.1	hypothetical protein	-
LXE91_RS41880 (LXE91_41785)	143044..143244	+	201	WP_034176085.1	hypothetical protein	-
LXE91_RS41885 (LXE91_41790)	143432..144085	+	654	WP_027813530.1	helix-turn-helix domain-containing protein	-
LXE91_RS41890 (LXE91_41795)	144106..144411	+	306	WP_027813531.1	hypothetical protein	-
LXE91_RS41895 (LXE91_41800)	144456..145541	+	1086	WP_027813532.1	hypothetical protein	-
LXE91_RS41900 (LXE91_41805)	145552..145782	+	231	WP_027813533.1	hypothetical protein	-
LXE91_RS41905 (LXE91_41810)	145792..146046	+	255	WP_046544062.1	hypothetical protein	-
LXE91_RS41910 (LXE91_41815)	146262..146933	+	672	WP_034176084.1	hypothetical protein	-
LXE91_RS41915 (LXE91_41820)	147015..147305	+	291	WP_027813562.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..199797	199797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14869.13 Da
• Isoelectric Point: 4.8604

>T231559 WP_027813527.1 NZ_CP090644:141926-142336 [Burkholderia contaminans]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIDANDMLIAAHALAVDAVLVTDNTAEFTRIAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T231559 NZ_CP126928:c4385169-4384996 [Escherichia coli O2:K1:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9375.64 Da
• Isoelectric Point: 4.6856

>AT231559 WP_027813526.1 NZ_CP090644:141687-141929 [Burkholderia contaminans]
MPNHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDEIFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT231559 NZ_CP126928:c4385493-4385197 [Escherichia coli O2:K1:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTCCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A6P2T8K5