Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 14181..14775 Replicon plasmid unnamed1
Accession NZ_CP090638
Organism Burkholderia contaminans strain FFI45

Toxin (Protein)


Gene name PumA Uniprot ID A0A228JB64
Locus tag LXE92_RS39075 Protein ID WP_027811055.1
Coordinates 14181..14474 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A228JAU5
Locus tag LXE92_RS39080 Protein ID WP_027811056.1
Coordinates 14476..14775 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXE92_RS39050 (LXE92_39005) 9567..11777 + 2211 WP_226251535.1 ParB/RepB/Spo0J family partition protein -
LXE92_RS39055 (LXE92_39010) 12818..13084 + 267 WP_027811052.1 hypothetical protein -
LXE92_RS39060 (LXE92_39015) 13059..13610 + 552 WP_077186813.1 hypothetical protein -
LXE92_RS39065 (LXE92_39020) 13655..13876 + 222 WP_027811054.1 DUF3717 domain-containing protein -
LXE92_RS39070 (LXE92_39025) 13933..14076 + 144 WP_154233621.1 hypothetical protein -
LXE92_RS39075 (LXE92_39030) 14181..14474 + 294 WP_027811055.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LXE92_RS39080 (LXE92_39035) 14476..14775 + 300 WP_027811056.1 putative addiction module antidote protein Antitoxin
LXE92_RS39085 (LXE92_39040) 14938..15249 - 312 WP_027811057.1 hypothetical protein -
LXE92_RS39090 (LXE92_39045) 15497..15997 - 501 WP_278135533.1 hypothetical protein -
LXE92_RS39095 (LXE92_39050) 17014..17415 + 402 WP_027811058.1 hypothetical protein -
LXE92_RS39100 (LXE92_39055) 17408..19474 + 2067 WP_027811059.1 type IV secretion system DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..198595 198595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(11-87)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10854.50 Da        Isoelectric Point: 10.6086

>T231547 WP_027811055.1 NZ_CP090638:14181-14474 [Burkholderia contaminans]
MRIEQTDDFAKWLRGLRDHIARAQIAKRIQRLARGQYGDMKPVGAGVSEMRVHVGPGYRVYFVQRGSTLVVLLCGGDKST
QQRDIERAIALSAQLGD

Download         Length: 294 bp

>T231547 NZ_CP126928:1929521-1929775 [Escherichia coli O2:K1:H4]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10716.01 Da        Isoelectric Point: 4.6364

>AT231547 WP_027811056.1 NZ_CP090638:14476-14775 [Burkholderia contaminans]
MGIKTTPFDAAEYLDTPEAQAEYLRISFETGDSADIQEAIGTVARARGMTQIARDAGVGRESLYKALDEHGNPEFATILR
VVRALGLTLTVTTPVHEHA

Download         Length: 300 bp

>AT231547 NZ_CP126928:1929273-1929524 [Escherichia coli O2:K1:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A228JB64

Antitoxin

Source ID Structure
AlphaFold DB A0A228JAU5

References