Detailed information of TA system TA231545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3101542..3102162
• Replicon: chromosome
• Accession: NZ_CP090636
• Organism: Burkholderia contaminans strain FFI45

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LXE92_RS31275
• Protein ID: WP_124471846.1
• Coordinates: 3101542..3101859 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1J9PWC2
• Locus tag: LXE92_RS31280
• Protein ID: WP_039344756.1
• Coordinates: 3101863..3102162 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXE92_RS31265 (LXE92_31205)	3098086..3099519	-	1434	WP_278131910.1	aldehyde dehydrogenase family protein	-
LXE92_RS31270 (LXE92_31210)	3099549..3101189	-	1641	WP_039344750.1	acetolactate synthase large subunit	-
LXE92_RS31275 (LXE92_31215)	3101542..3101859	+	318	WP_124471846.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXE92_RS31280 (LXE92_31220)	3101863..3102162	+	300	WP_039344756.1	putative addiction module antidote protein	Antitoxin
LXE92_RS31285 (LXE92_31225)	3102211..3103497	-	1287	WP_278131911.1	DUF445 domain-containing protein	-
LXE92_RS31290 (LXE92_31230)	3103668..3104888	-	1221	WP_278131912.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
LXE92_RS31295 (LXE92_31235)	3104878..3105204	-	327	WP_039344765.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
LXE92_RS31300 (LXE92_31240)	3105225..3105713	-	489	WP_011355917.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
LXE92_RS31305 (LXE92_31245)	3105728..3106999	-	1272	WP_039344770.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11868.59 Da
• Isoelectric Point: 9.5691

>T231545 WP_124471846.1 NZ_CP090636:3101542-3101859 [Burkholderia contaminans]
MPYSAPTFSIRTTDVFDTWFAGLQDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T231545 NZ_CP126928:1103223-1103558 [Escherichia coli O2:K1:H4]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10649.01 Da
• Isoelectric Point: 6.3217

>AT231545 WP_039344756.1 NZ_CP090636:3101863-3102162 [Burkholderia contaminans]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGSKAHA

Nucleotide

• Length: 300 bp

>AT231545 NZ_CP126928:1102975-1103223 [Escherichia coli O2:K1:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1J9PWC2