Detailed information of TA system TA231543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2363117..2363750
• Replicon: chromosome
• Accession: NZ_CP090636
• Organism: Burkholderia contaminans strain FFI45

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LXE92_RS28045
• Protein ID: WP_278131276.1
• Coordinates: 2363117..2363530 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9P9A2
• Locus tag: LXE92_RS28050
• Protein ID: WP_071333622.1
• Coordinates: 2363517..2363750 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXE92_RS28025 (LXE92_27960)	2358792..2360096	+	1305	WP_105815253.1	shikimate transporter	-
LXE92_RS28030 (LXE92_27965)	2360314..2361615	+	1302	WP_039367440.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
LXE92_RS28035 (LXE92_27970)	2361703..2362314	-	612	WP_278131274.1	hypothetical protein	-
LXE92_RS28040 (LXE92_27975)	2362554..2362967	+	414	WP_278131275.1	hypothetical protein	-
LXE92_RS28045 (LXE92_27980)	2363117..2363530	-	414	WP_278131276.1	PIN domain-containing protein	Toxin
LXE92_RS28050 (LXE92_27985)	2363517..2363750	-	234	WP_071333622.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LXE92_RS28055 (LXE92_27990)	2363917..2364687	+	771	WP_039367494.1	IclR family transcriptional regulator C-terminal domain-containing protein	-
LXE92_RS28060 (LXE92_27995)	2364742..2367041	-	2300	Protein_2071	ATP-dependent DNA helicase	-
LXE92_RS28065 (LXE92_28000)	2367038..2368732	-	1695	WP_278131279.1	VRR-NUC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15160.40 Da
• Isoelectric Point: 4.7627

>T231543 WP_278131276.1 NZ_CP090636:c2363530-2363117 [Burkholderia contaminans]
MSGIDATKSFVDSNVVLYLLSEDETKADRAESLLLRRPTISVQVLNEVASVCSRKLRMPWIDVGQFLEPIRSLCRVVPIS
VDTHDLGRKLAERHGLSFYDACIVASASIEGCEYLYTEDMHDGLCVEGGPVLKNPFA

Nucleotide

• Length: 414 bp

>T231543 NZ_CP126928:c832124-831747 [Escherichia coli O2:K1:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8804.10 Da
• Isoelectric Point: 8.8306

>AT231543 WP_071333622.1 NZ_CP090636:c2363750-2363517 [Burkholderia contaminans]
MLVAKWGNSLAVRLPASVVDALELREGDDIEIVVDSPRVFAVSRKPRPEELLQRLRRFRGKLPADFKFGRDDANERD

Nucleotide

• Length: 234 bp

>AT231543 NZ_CP126928:c832548-832171 [Escherichia coli O2:K1:H4]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9P9A2