Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4778004..4778814 Replicon chromosome
Accession NZ_CP090626
Organism Klebsiella pneumoniae isolate KpBO_TO3

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag L0104_RS23855 Protein ID WP_002887280.1
Coordinates 4778004..4778537 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L0104_RS23860 Protein ID WP_002887278.1
Coordinates 4778548..4778814 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0104_RS23850 (L0104_23850) 4776835..4777956 + 1122 WP_002887282.1 cupin domain-containing protein -
L0104_RS23855 (L0104_23855) 4778004..4778537 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
L0104_RS23860 (L0104_23860) 4778548..4778814 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L0104_RS23865 (L0104_23865) 4778917..4780350 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
L0104_RS23870 (L0104_23870) 4780340..4781023 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L0104_RS23875 (L0104_23875) 4781195..4782580 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
L0104_RS23880 (L0104_23880) 4782598..4782942 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T231502 WP_002887280.1 NZ_CP090626:c4778537-4778004 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T231502 NZ_CP126681:1233815-1233991 [Pantoea agglomerans]
GTGAAGCAGAGCGAGTTCAGGCGGTGGCTTGAATCTCAGGGAGTCGAAGTTTCAAACGGTACTAACCATCTGAAGCTGAG
ATACAACGGGAAGCGAAGTGTAATGCCGAGGCATCCCGGCGCTGAGTTAAAAGAACCACTGCGAAAGGCCATAATGAAGC
AGTTAGGCCTGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT231502 WP_002887278.1 NZ_CP090626:c4778814-4778548 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT231502 NZ_CP126681:1234040-1234447 [Pantoea agglomerans]
ATGCGATACCCTATTAATCTCGAGCCGTGCGACGGTGGATATGTGGTTTCGTTCCCGGATATCCCGGAGGCGCTTACTCA
GGGCGATACGCGTGAGGAAGCGTTAGAGATGGGGCTGGATGCGCTGGTTACTTCATTTGATTTCTATTTTGAAGATAACC
AGCCAGTTCCGGCACCGGGTCCGATTACCGGCGATTTTGTAGAGGTTCCGGCGAGTGTGTCGGCAAAGGTGCTGTTGCTA
AATGCTTTCCTTGCTTCCGGCTTAACTCAGGTTGAGCTGGCTTCACGCATGGGAGTTAAGAAGCAGGAAGTGACACGCAT
CTTCGATCTTCACCACTCGACCAAAATAGATACGGTTCAAAAAGCGCTGAATGCGCTGGGTAAGCGGCTTGAATTAGTCG
CTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References