Detailed information of TA system TA023148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2153896..2154367
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: ECH74115_RS11670
• Protein ID: WP_001303511.1
• Coordinates: 2154089..2154367 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: ECH74115_RS11665
• Protein ID: WP_001302048.1
• Coordinates: 2153896..2154087 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS11620	2149103..2149687	-	585	WP_000206793.1	DUF551 domain-containing protein	-
ECH74115_RS11625	2149744..2150139	-	396	WP_001118159.1	DUF977 family protein	-
ECH74115_RS11630	2150155..2150924	-	770	Protein_2101	DUF1627 domain-containing protein	-
ECH74115_RS11640	2150950..2151690	-	741	WP_000788938.1	ATP-binding protein	-
ECH74115_RS11645	2151697..2152659	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
ECH74115_RS11650	2152682..2153107	-	426	WP_000693943.1	Rha family transcriptional regulator	-
ECH74115_RS11655	2153104..2153406	-	303	WP_000172738.1	transcriptional regulator	-
ECH74115_RS32810	2153504..2153875	+	372	WP_001169686.1	hypothetical protein	-
ECH74115_RS11665	2153896..2154087	+	192	WP_001302048.1	hypothetical protein	Antitoxin
ECH74115_RS11670	2154089..2154367	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECH74115_RS11680	2154720..2155019	+	300	WP_001240334.1	hypothetical protein	-
ECH74115_RS11685	2155091..2155309	+	219	WP_001171930.1	hypothetical protein	-
ECH74115_RS30980	2155878..2156066	+	189	WP_000449192.1	cell division inhibition protein DicB	-
ECH74115_RS11690	2156063..2156254	+	192	WP_001090200.1	DUF1482 family protein	-
ECH74115_RS11695	2156347..2157432	+	1086	Protein_2113	exonuclease	-
ECH74115_RS11705	2157483..2158696	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2075432..2163590	88158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T23148 WP_001303511.1 NC_011353:2154089-2154367 [Escherichia coli O157:H7 str. EC4115]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T23148 NC_011353:2154089-2154367 [Escherichia coli O157:H7 str. EC4115]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT23148 WP_001302048.1 NC_011353:2153896-2154087 [Escherichia coli O157:H7 str. EC4115]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT23148 NC_011353:2153896-2154087 [Escherichia coli O157:H7 str. EC4115]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0