Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5253955..5254580 Replicon chromosome
Accession NZ_CP090622
Organism Klebsiella pneumoniae strain KP19-3138

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag LXT11_RS28275 Protein ID WP_019705794.1
Coordinates 5253955..5254338 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LXT11_RS28280 Protein ID WP_004150355.1
Coordinates 5254338..5254580 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXT11_RS28260 (5251321) 5251321..5252223 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LXT11_RS28265 (5252220) 5252220..5252855 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LXT11_RS28270 (5252852) 5252852..5253781 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LXT11_RS28275 (5253955) 5253955..5254338 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
LXT11_RS28280 (5254338) 5254338..5254580 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LXT11_RS28285 (5254785) 5254785..5255702 + 918 WP_004146235.1 alpha/beta hydrolase -
LXT11_RS28290 (5255717) 5255717..5256658 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LXT11_RS28295 (5256703) 5256703..5257140 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LXT11_RS28300 (5257137) 5257137..5257997 - 861 WP_004146232.1 virulence factor BrkB family protein -
LXT11_RS28305 (5257991) 5257991..5258590 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T231468 WP_019705794.1 NZ_CP090622:c5254338-5253955 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T231468 NZ_CP126537:c14264-13914 [Citrobacter freundii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT231468 WP_004150355.1 NZ_CP090622:c5254580-5254338 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT231468 NZ_CP126537:c13909-13607 [Citrobacter freundii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References