Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4636238..4637048 Replicon chromosome
Accession NZ_CP090622
Organism Klebsiella pneumoniae strain KP19-3138

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LXT11_RS25405 Protein ID WP_004178461.1
Coordinates 4636238..4636771 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LXT11_RS25410 Protein ID WP_002887278.1
Coordinates 4636782..4637048 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXT11_RS25400 (4635069) 4635069..4636190 + 1122 WP_004186850.1 cupin domain-containing protein -
LXT11_RS25405 (4636238) 4636238..4636771 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LXT11_RS25410 (4636782) 4636782..4637048 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LXT11_RS25415 (4637151) 4637151..4638584 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
LXT11_RS25420 (4638574) 4638574..4639257 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LXT11_RS25425 (4639430) 4639430..4640815 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
LXT11_RS25430 (4640833) 4640833..4641177 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T231465 WP_004178461.1 NZ_CP090622:c4636771-4636238 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T231465 NZ_CP126536:c4814936-4814634 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACTAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT231465 WP_002887278.1 NZ_CP090622:c4637048-4636782 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT231465 NZ_CP126536:c4814607-4814266 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References