Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1939640..1940278 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECH74115_RS10480 Protein ID WP_000813794.1
Coordinates 1939640..1939816 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECH74115_RS10485 Protein ID WP_001270286.1
Coordinates 1939862..1940278 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS10460 1935261..1936434 - 1174 Protein_1879 BenE family transporter YdcO -
ECH74115_RS10465 1936526..1937062 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ECH74115_RS10470 1937135..1939096 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECH74115_RS10475 1939188..1939418 - 231 WP_000494244.1 YncJ family protein -
ECH74115_RS10480 1939640..1939816 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECH74115_RS10485 1939862..1940278 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECH74115_RS10490 1940357..1941763 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ECH74115_RS10495 1942008..1943153 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ECH74115_RS10500 1943171..1944184 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ECH74115_RS10505 1944185..1945126 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T23145 WP_000813794.1 NC_011353:1939640-1939816 [Escherichia coli O157:H7 str. EC4115]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T23145 NC_011353:1939640-1939816 [Escherichia coli O157:H7 str. EC4115]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT23145 WP_001270286.1 NC_011353:1939862-1940278 [Escherichia coli O157:H7 str. EC4115]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT23145 NC_011353:1939862-1940278 [Escherichia coli O157:H7 str. EC4115]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References