Detailed information of TA system TA231446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26482..27007
• Replicon: plasmid pKP19-3138-3
• Accession: NZ_CP090619
• Organism: Klebsiella pneumoniae strain KP19-3138

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LXT11_RS01230
• Protein ID: WP_013023785.1
• Coordinates: 26702..27007 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LXT11_RS01225
• Protein ID: WP_001568025.1
• Coordinates: 26482..26700 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXT11_RS01205 (LXT11_01205)	21729..22697	+	969	WP_077253318.1	IS5 family transposase	-
LXT11_RS01210 (LXT11_01210)	23169..23960	-	792	WP_221928726.1	ribbon-helix-helix domain-containing protein	-
LXT11_RS01215 (LXT11_01215)	24143..25171	-	1029	WP_032445668.1	Abi family protein	-
LXT11_RS01220 (LXT11_01220)	25332..25913	-	582	WP_072310991.1	hypothetical protein	-
LXT11_RS01225 (LXT11_01225)	26482..26700	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LXT11_RS01230 (LXT11_01230)	26702..27007	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LXT11_RS01235 (LXT11_01235)	27176..27571	+	396	WP_017899885.1	hypothetical protein	-
LXT11_RS01240 (LXT11_01240)	27598..27921	+	324	WP_004197641.1	hypothetical protein	-
LXT11_RS01245 (LXT11_01245)	27918..28934	+	1017	WP_118842534.1	hypothetical protein	-
LXT11_RS01250 (LXT11_01250)	29132..29917	+	786	WP_046664219.1	site-specific integrase	-
LXT11_RS01255 (LXT11_01255)	30366..31121	+	756	WP_001568031.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-8	-	1..102938	102938
	-	flank	IS/Tn	mcr-8	-	15672..22697	7025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T231446 WP_013023785.1 NZ_CP090619:26702-27007 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T231446 NZ_CP126525:2312318-2313058 [Bacillus toyonensis]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTTTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTATGAAGAGAAAAAGCAGCGAATTCGAAAAACTTGGTATGGATTATTCATAACATCAGTCACAGTTTATT
TCATGATTAAAGGAATCGATTTAACCCTCTGGAAAAATTTATTAATGTTCACTGCGATGGTCATTTTCGTTGATATTTCA
TTTATTTTAACACCTAACATTTCGGAAATATGGGGAGCGAAATTTAGCGATATCGGTAAAACTGTTCAATCCATAAAGCG
GTCATTAATCGCTTCAAAAGCAAGAGGGGAAATATATACGAACATTATCCAAAATATTAATCCAGCAGCATTTGGGACGA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAAATCGGAGCGAAA
ATTGTTGTATTTGAAGCAGCGAATGAATTAAATACGGGCTTTCGTGGTATTCGTTCTCAATTTAGTATTACAATACCTAT
AGAGCATATAGAGCAATTGAATGAACAAAAAGCAGTACAAGTAGAAAACGTGGGAATTATACCAGCAAAAATAATGAATG
ATGTTTTTATCGTTATTGATGGGAAGAAGAATAGCCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTATTTTAGTAAGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT231446 WP_001568025.1 NZ_CP090619:26482-26700 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT231446 NZ_CP126525:2313171-2313296 [Bacillus toyonensis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCTTTAATCAAAGGAGATAC
GTTGCAGCAAGTAGAAAAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8