Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 57329..58065 Replicon plasmid pKP19-3138-2
Accession NZ_CP090618
Organism Klebsiella pneumoniae strain KP19-3138

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LXT11_RS00305 Protein ID WP_003026803.1
Coordinates 57583..58065 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LXT11_RS00300 Protein ID WP_003026799.1
Coordinates 57329..57595 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXT11_RS00255 (LXT11_00255) 52603..52953 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LXT11_RS00260 (LXT11_00260) 53311..53580 + 270 WP_004152102.1 hypothetical protein -
LXT11_RS00265 (LXT11_00265) 53568..54143 + 576 WP_004152103.1 hypothetical protein -
LXT11_RS00270 (LXT11_00270) 54174..54668 + 495 WP_004152104.1 DNA-binding protein -
LXT11_RS00275 (LXT11_00275) 54712..55080 + 369 WP_004152105.1 hypothetical protein -
LXT11_RS00280 (LXT11_00280) 55114..55317 + 204 WP_004152106.1 HHA domain-containing protein -
LXT11_RS00285 (LXT11_00285) 55366..55623 + 258 WP_004152107.1 hypothetical protein -
LXT11_RS00290 (LXT11_00290) 55919..56899 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
LXT11_RS00295 (LXT11_00295) 56968..57153 + 186 Protein_58 hypothetical protein -
LXT11_RS00300 (LXT11_00300) 57329..57595 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LXT11_RS00305 (LXT11_00305) 57583..58065 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LXT11_RS00310 (LXT11_00310) 58266..59669 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LXT11_RS00315 (LXT11_00315) 59698..60330 - 633 WP_001567369.1 hypothetical protein -
LXT11_RS00320 (LXT11_00320) 60544..60810 + 267 Protein_63 ISNCY family transposase -
LXT11_RS00325 (LXT11_00325) 60900..60995 + 96 Protein_64 IS3 family transposase -
LXT11_RS00330 (LXT11_00330) 61971..62939 + 969 WP_221692532.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / qnrS1 - 1..208364 208364
- inside IScluster/Tn - - 55919..68280 12361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T231445 WP_003026803.1 NZ_CP090618:57583-58065 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T231445 NZ_CP126525:238865-239215 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT231445 WP_003026799.1 NZ_CP090618:57329-57595 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT231445 NZ_CP126525:238573-238860 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References