Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3932427..3932924 Replicon chromosome
Accession NZ_CP090611
Organism Escherichia coli isolate DCE7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LZG50_RS19435 Protein ID WP_001173343.1
Coordinates 3932427..3932600 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag LZG50_RS19440 Protein ID WP_001312331.1
Coordinates 3932628..3932924 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG50_RS19425 (3929097) 3929097..3930554 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
LZG50_RS19430 (3930791) 3930791..3932308 + 1518 WP_000003806.1 lysine--tRNA ligase -
LZG50_RS19435 (3932427) 3932427..3932600 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LZG50_RS19440 (3932628) 3932628..3932924 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LZG50_RS19445 (3933152) 3933152..3933424 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LZG50_RS19450 (3933436) 3933436..3933666 - 231 WP_000371709.1 4Fe-4S mono-cluster protein YjdI -
LZG50_RS19455 (3933847) 3933847..3935478 + 1632 WP_001216466.1 sensor histidine kinase -
LZG50_RS19460 (3935475) 3935475..3936194 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T231427 WP_001173343.1 NZ_CP090611:c3932600-3932427 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T231427 NZ_CP126349:22756-23037 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT231427 WP_001312331.1 NZ_CP090611:c3932924-3932628 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT231427 NZ_CP126349:22515-22766 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References