Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3540078..3540610 Replicon chromosome
Accession NZ_CP090611
Organism Escherichia coli isolate DCE7

Toxin (Protein)


Gene name ccdB Uniprot ID A0A2A7M2E8
Locus tag LZG50_RS17415 Protein ID WP_001160959.1
Coordinates 3540078..3540374 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LZG50_RS17420 Protein ID WP_000125566.1
Coordinates 3540377..3540610 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG50_RS17390 (3535753) 3535753..3537039 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
LZG50_RS17395 (3537039) 3537039..3538028 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LZG50_RS17400 (3538025) 3538025..3538846 + 822 WP_001065367.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LZG50_RS17405 (3538849) 3538849..3539226 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LZG50_RS17410 (3539233) 3539233..3540081 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LZG50_RS17415 (3540078) 3540078..3540374 - 297 WP_001160959.1 CcdB family protein Toxin
LZG50_RS17420 (3540377) 3540377..3540610 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LZG50_RS17425 (3540696) 3540696..3541175 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
LZG50_RS17430 (3541367) 3541367..3543229 - 1863 WP_096891018.1 glutathione-regulated potassium-efflux system protein KefC -
LZG50_RS17435 (3543222) 3543222..3543752 - 531 WP_000600723.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LZG50_RS17440 (3543860) 3543860..3545191 - 1332 WP_001183198.1 MFS transporter -
LZG50_RS17445 (3545250) 3545250..3545537 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-97)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11089.98 Da        Isoelectric Point: 8.0406

>T231424 WP_001160959.1 NZ_CP090611:c3540374-3540078 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPFIRIRPPERLNTILLLVDGKEYVLMTHETETVPVNALGTKFCDA
SAHRTLIKGALDFMLDGI

Download         Length: 297 bp

>T231424 NZ_CP126348:c4038248-4037898 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT231424 WP_000125566.1 NZ_CP090611:c3540610-3540377 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT231424 NZ_CP126348:c4038493-4038242 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A7M2E8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References