Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3097368..3097986 Replicon chromosome
Accession NZ_CP090611
Organism Escherichia coli isolate DCE7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LZG50_RS15310 Protein ID WP_001291435.1
Coordinates 3097768..3097986 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LZG50_RS15305 Protein ID WP_000344800.1
Coordinates 3097368..3097742 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG50_RS15295 (3092459) 3092459..3093652 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LZG50_RS15300 (3093675) 3093675..3096824 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LZG50_RS15305 (3097368) 3097368..3097742 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LZG50_RS15310 (3097768) 3097768..3097986 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LZG50_RS15315 (3098160) 3098160..3098711 + 552 WP_000102539.1 maltose O-acetyltransferase -
LZG50_RS15320 (3098827) 3098827..3099297 + 471 WP_000136192.1 YlaC family protein -
LZG50_RS15325 (3099461) 3099461..3101011 + 1551 WP_014640107.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LZG50_RS15330 (3101053) 3101053..3101406 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LZG50_RS15340 (3101785) 3101785..3102096 + 312 WP_000409908.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3102122..3103495 1373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T231423 WP_001291435.1 NZ_CP090611:3097768-3097986 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T231423 NZ_CP126348:c3966504-3966163 [Escherichia coli]
ATGAAACCTCAACCTGCAACAACTTCGCGGGCGGTAAAGCCTTGTCTGTCGCCCGTGGCAGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGACGCTAAACGACACGCCGTTCAGTGATGAAACTGTGATTAAGGAACATATCG
ATGCCGGTATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATATGAACTAGTTCGTATCGATCGCAGAGGGTTT
AACTGGCAGGAGCAATCACCTTATCTTCGCGCGGTAGATATACTGCGAGCACGACGGGCGATTGGTTTATTGCGACGAAG
TCTTAATGATGCTGTATTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT231423 WP_000344800.1 NZ_CP090611:3097368-3097742 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT231423 NZ_CP126348:c3966842-3966525 [Escherichia coli]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATCACGCCACACCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGGCT
GCATTATTTGGCTGACCGGGCCAGCATCATCGGTAAGTTCAGTGGCGCCGAATGCCGGAAGCTGGATGAAATATTCCCGC
ACTTTATCAGCCAGATGGAATCCATGCTGACCACGGGAGAACTGAATCCCCGTTACACCCACTGTATTACCCTGTATCAC
AATGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCAATCAGCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References