Detailed information of TA system TA231422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2401106..2401890
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LZG50_RS11825
• Protein ID: WP_000613626.1
• Coordinates: 2401396..2401890 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: LZG50_RS11820
• Protein ID: WP_000718446.1
• Coordinates: 2401106..2401399 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS11810 (2396267)	2396267..2397226	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LZG50_RS11815 (2397799)	2397799..2400972	+	3174	WP_096841489.1	ribonuclease E	-
LZG50_RS11820 (2401106)	2401106..2401399	+	294	WP_000718446.1	DUF1778 domain-containing protein	Antitoxin
LZG50_RS11825 (2401396)	2401396..2401890	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LZG50_RS11830 (2401985)	2401985..2402938	-	954	WP_022645524.1	flagellar hook-associated protein FlgL	-
LZG50_RS11835 (2402950)	2402950..2404593	-	1644	WP_032171110.1	flagellar hook-associated protein FlgK	-
LZG50_RS11840 (2404659)	2404659..2405600	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LZG50_RS11845 (2405600)	2405600..2406697	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T231422 WP_000613626.1 NZ_CP090611:2401396-2401890 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T231422 NZ_CP126348:3574409-3574687 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11053.63 Da
• Isoelectric Point: 10.5423

>AT231422 WP_000718446.1 NZ_CP090611:2401106-2401399 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT231422 NZ_CP126348:3574146-3574406 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9