Detailed information of TA system TA231419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1961426..1963014
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LZG50_RS09540
• Protein ID: WP_096841321.1
• Coordinates: 1961692..1963014 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: LZG50_RS09535
• Protein ID: WP_001296076.1
• Coordinates: 1961426..1961692 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS09520 (1957793)	1957793..1959244	+	1452	WP_000854629.1	tagaturonate reductase	-
LZG50_RS09525 (1959441)	1959441..1960355	+	915	WP_096841323.1	bestrophin family protein	-
LZG50_RS09530 (1960359)	1960359..1961117	-	759	WP_096841322.1	trans-aconitate 2-methyltransferase	-
LZG50_RS09535 (1961426)	1961426..1961692	+	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LZG50_RS09540 (1961692)	1961692..1963014	+	1323	WP_096841321.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LZG50_RS09545 (1963230)	1963230..1963874	+	645	WP_096891228.1	pentapeptide repeat-containing protein	-
LZG50_RS09550 (1964249)	1964249..1964506	-	258	Protein_1870	helix-turn-helix domain-containing protein	-
LZG50_RS09555 (1964759)	1964759..1966120	+	1362	WP_047668719.1	site-specific integrase	-
LZG50_RS09560 (1966177)	1966177..1967667	+	1491	WP_234732927.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49329.96 Da
• Isoelectric Point: 7.7906

>T231419 WP_096841321.1 NZ_CP090611:1961692-1963014 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAVEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRGRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENLVTAVETNVLRLHDRLSREYGIK

Nucleotide

• Length: 1323 bp

>T231419 NZ_CP126348:2604425-2604532 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT231419 WP_001296076.1 NZ_CP090611:1961426-1961692 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT231419 NZ_CP126348:c2604375-2604312 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A061YNW4