Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1001959..1002542 Replicon chromosome
Accession NZ_CP090611
Organism Escherichia coli isolate DCE7

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag LZG50_RS04870 Protein ID WP_000254750.1
Coordinates 1002207..1002542 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LZG50_RS04865 Protein ID WP_000581937.1
Coordinates 1001959..1002207 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG50_RS04855 (998298) 998298..999599 + 1302 WP_096891133.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
LZG50_RS04860 (999647) 999647..1001881 + 2235 WP_000226815.1 GTP pyrophosphokinase -
LZG50_RS04865 (1001959) 1001959..1002207 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LZG50_RS04870 (1002207) 1002207..1002542 + 336 WP_000254750.1 endoribonuclease MazF Toxin
LZG50_RS04875 (1002614) 1002614..1003405 + 792 WP_027662693.1 nucleoside triphosphate pyrophosphohydrolase -
LZG50_RS04880 (1003633) 1003633..1005270 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LZG50_RS04885 (1005358) 1005358..1006656 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T231417 WP_000254750.1 NZ_CP090611:1002207-1002542 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T231417 NZ_CP126348:2603354-2603461 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT231417 WP_000581937.1 NZ_CP090611:1001959-1002207 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT231417 NZ_CP126348:c2603307-2603241 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References